1) asymmetric PCR
非对称PCR
1.
We applied the technology of systematic evolution of ligands by exponential enrichment(SELEX), separated ssDNA ligand by means of interaction between random oligonucleotide library and BAFF and then obtained ssDNA aptamers binding recombinant human BAFF through symmetric PCR and asymmetric PCR amplification.
应用指数方式富集的配体系统进化(SELEX)技术,将随机寡核苷酸文库与B淋巴细胞活化因子相互作用,分离出结合的寡核苷酸配体,优化对称和非对称PCR实验条件,成功进行了13轮次进化筛选,并将进化产物克隆,随机选取42个阳性克隆测序,对测得的41序列根据同源性分为6类不同序列,应用斑点印迹法证实6类序列均与B淋巴细胞活化因子相结合;酶联法比较各适体序列对B淋巴细胞活化因子的相对结合力,对光密度值进行统计学分析,以序列44光密度值0。
2) Asymmetric PCR
不对称PCR
1.
Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR;
分子信标用于不对称PCR检测乙型肝炎病毒
2.
Methods Different ways of sample preparation were observed based on HLA-A biochip, such as two different genome DNA extraction methods (Phenol-chloroform method and leucocyte lysis method), nano-magnetic beads (Fe 2O 3) used in nucleus cell separation, the primers concentration and ratio in asymmetric PCR and three PCR product purification ways.
方法 观察了样品制备方法 (传统酚 氯仿法、白细胞裂解液法、Fe2 O3 纳米磁珠 )、不对称PCR制备ssDNA的参数、PCR产物纯化方法对杂交结果的影响。
3.
By means of asymmetric PCR and indirect fl.
采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记,标记样品与寡核苷酸芯片杂交后,进行芯片清洗、扫描及结果分析。
3) asymmetry PCR
不对称PCR
1.
Two different PCR methods,seminested symmetry PCR and seminested asymmetry PCR,were adopted respectively.
分别采用对称PCR和不对称PCR扩增,均可得到扩增的目的片段。
4) symmetric PCR
对称PCR
1.
We applied the technology of systematic evolution of ligands by exponential enrichment(SELEX), separated ssDNA ligand by means of interaction between random oligonucleotide library and BAFF and then obtained ssDNA aptamers binding recombinant human BAFF through symmetric PCR and asymmetric PCR amplification.
应用指数方式富集的配体系统进化(SELEX)技术,将随机寡核苷酸文库与B淋巴细胞活化因子相互作用,分离出结合的寡核苷酸配体,优化对称和非对称PCR实验条件,成功进行了13轮次进化筛选,并将进化产物克隆,随机选取42个阳性克隆测序,对测得的41序列根据同源性分为6类不同序列,应用斑点印迹法证实6类序列均与B淋巴细胞活化因子相结合;酶联法比较各适体序列对B淋巴细胞活化因子的相对结合力,对光密度值进行统计学分析,以序列44光密度值0。
5) TAIL-PCR
温度非对称交互PCR
1.
Unfortunately, in spite of the fact that TAIL_PCR technique has been expanded vastly and adopted in transposon mutagenesis in rice, a reliable, highly reproducible TAIL-PCR procedure especially for rice genomic DNA is still not available, mainly due to the complexity of rice genome .
温度非对称交互PCR(TAIL PCR)技术已广泛应用于从多种生物体系克隆侧翼于已知序列的DNA片段的分子操作中 ,并极大地促进了反向遗传学研究。
6) thermal asymmetric interlaced PCR (TAIL-PCR)
热不对称PCR(TAIL-PCR)
1.
oryzicola and the critical pathogen of bacterial leaf streak in rice (Oryza sativa), thermal asymmetric interlaced PCR (TAIL-PCR) and Tn5 transposon rescue were adopted to verify 8 virulence-reduced mutants screened from the Tn5 inserted library of the pathogen based on virulence assay in rice.
为快速准确地鉴定Tn5的插入位点和相应的功能基因,研究采用热不对称PCR(TAIL-PCR)和Tn5转座子质粒拯救两种技术,鉴别了在筛选水稻条斑病菌(Xanthomonas oryzaepv。
补充资料：对称与非对称
反映客观事物在结构、功能、时空上的特殊联系的范畴。对称指事物以一定的中介进行某种变化时出现的不变性，非对称指事物以一定的中介进行某种变化时出现的可变性。在自然界中普遍存在，形式多样。对称有空间对称(包括形象对称和结构对称)、时间对称、概念对称等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条