1) RACE
[英][reɪs] [美][res]
cDNA末端快速扩增技术
1.
To understand the mechanism of chick focal adhesion kinase gene(FAK)expression and regulation,the transcription start sites and four kinds alternatively spliced 5′end(5′untranslated region,5′UTR)of FAK mRNA in chick embryo fibroblast cells were identified by RACE.
为了研究鸡局部黏着斑激酶(FAK)基因表达调控的分子机制,运用cDNA末端快速扩增技术确定了鸡胚成纤维细胞FAK基因的转录起始位点,并发现FAK基因mRNA的5′非翻译区(5′UTR)存在4种剪切形式。
2.
Rapid amplification of cDNA ends (RACE) is a polymerase chain reaction (PCR)-based technique which was developed to facilitate the cloning of full-length cDNA 5 and 3 ends after a partial cDNA sequence has been obrained by other methods.
cDNA末端快速扩增技术是一种基于多聚酶链式反应的技术 ,它的发展大大便利了应用其它方法获得的部分cDNA序列后克隆全长cDNA 5’和 3’末端的工作。
3.
This study has cloned Grass carp The full-length cDNA of CRP by the methods of RACE and analysed the sequences, And the CRP tissue distribution was detected in the Grass carp different tissue With the method of RT-PCR,as well as Prokaryotic Expression vector of CRP was suceessfully to be constructed.
本研究采用cDNA末端快速扩增技术(RACE)’对草鱼C反应蛋白基因的cDNA全长序列进行克隆,序列分析,并通过RT-PCR方法研究了草鱼CRP组织分布,同时成功构建了草鱼CRP原核表达重组质粒。
2) RACE
[英][reɪs] [美][res]
快速cDNA末端扩增
1.
The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).
利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
3) RACE
[英][reɪs] [美][res]
cDNA末端快速扩增
1.
METHODS: The full-length cDNA of rabbit BK_Ca beta subunit gene was amplified by 3′ RACE, 5′ RACE and RT-PCR from rabbit sphincter of Oddi (SO).
方法:采用3′及5′cDNA末端快速扩增(RACE)及RT-PCR技术从兔Oddi括约肌(SO)组织获得BKCa通道β亚基的全长cDNA序列。
2.
To find a novel gene which is related to protein of bovine seminal plasma (BSP) in human and study the new gene′s structural feature,expression and location in chromosome,the cDNA was cloned by using the 3′ rapid amplification of cDNA ends (RACE) and 5′ RACE methods and cloned into the expression vector of GST fusion protein (PGEX-5X-1),finally expressed in E.
采用cDNA末端快速扩增 (RACE)技术 ,克隆了一个BSP蛋白相关基因的cDNA序列 。
3.
Special primers were designed based on the sequence information of ferritin genes reported in the literature and used for rapid amplification of cDNA ends(RACE) for cloning Phascoloma esculenta ferritin gene.
根据已报道的铁结合蛋白基因的保守序列设计引物,用cDNA末端快速扩增(RACE)法,成功地从可口革囊星虫体液中获得铁结合蛋白基因的全长序列。
4) rapid amplification of cDNA end
cDNA末端快速扩增
1.
Methods: According to the partial sequences deposited in GenBank,the full-length cDNA was obtained using the techniques named rapid amplification of cDNA ends,and then,the sequence was analyzed bioinformatically.
方法:根据GenBank中已发表的NRDR的cDNA部分序列信息,运用cDNA末端快速扩增技术得到全长cDNA,然后利用生物信息学的方法对其进行分析。
5) rapid amplification of cDNA ends
cDNA末端快速扩增
1.
MATERIAL AND METHODS: Based on the rapid amplification of cDNA ends(RACE)method,gradient and nested PCR protocols were used to obtain full_length cDNA in order to get .
本研究应用cDNA末端快速扩增法(rapidamplificationofcDNAends,RACE)扩增此未知基因的全长,以进行下一步的基因功能研究。
6) rapid amplification of cDNA ends (RACE)
cDNA末端快速扩增
补充资料：核酸体外扩增技术
分子式：
CAS号：
性质：又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性，分解为单链。在较低温度(37～63℃)与引物退火：然后变温到72℃左右，在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链，然后又变性→退火→延伸反复进行。引物大大过量，dNTP过量，酶在高温中是较稳定的，这样经过几十个循环，目标DNA片段就按2n数递增。用此法可从mRNA中，cDNA库中扩增出目标基因。过去一般合成500～1000bp较好，现已发展出大片段(75kb)的PCR，且差错甚少。PCR技术的发展还可用于定点突变，质粒重组及FLP等方面。这一技术的作用范围日益扩大，已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破，已在分子生物学、医学、法学等领域发挥了极大的作用。
CAS号：
性质：又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性，分解为单链。在较低温度(37～63℃)与引物退火：然后变温到72℃左右，在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链，然后又变性→退火→延伸反复进行。引物大大过量，dNTP过量，酶在高温中是较稳定的，这样经过几十个循环，目标DNA片段就按2n数递增。用此法可从mRNA中，cDNA库中扩增出目标基因。过去一般合成500～1000bp较好，现已发展出大片段(75kb)的PCR，且差错甚少。PCR技术的发展还可用于定点突变，质粒重组及FLP等方面。这一技术的作用范围日益扩大，已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破，已在分子生物学、医学、法学等领域发挥了极大的作用。
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