1) 5'rapid amplification of cDNA ends
5'末端快速扩增
2) 5-RACE
5'-cDNA末端快速扩增
3) rapid amplification of 3' and 5' terminal regions
3'和5'末端快速扩增法
4) RACE
[英][reɪs] [美][res]
快速cDNA末端扩增
1.
The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).
利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
5) RACE
[英][reɪs] [美][res]
cDNA末端快速扩增
1.
METHODS: The full-length cDNA of rabbit BK_Ca beta subunit gene was amplified by 3′ RACE, 5′ RACE and RT-PCR from rabbit sphincter of Oddi (SO).
方法:采用3′及5′cDNA末端快速扩增(RACE)及RT-PCR技术从兔Oddi括约肌(SO)组织获得BKCa通道β亚基的全长cDNA序列。
2.
To find a novel gene which is related to protein of bovine seminal plasma (BSP) in human and study the new gene′s structural feature,expression and location in chromosome,the cDNA was cloned by using the 3′ rapid amplification of cDNA ends (RACE) and 5′ RACE methods and cloned into the expression vector of GST fusion protein (PGEX-5X-1),finally expressed in E.
采用cDNA末端快速扩增 (RACE)技术 ,克隆了一个BSP蛋白相关基因的cDNA序列 。
3.
Special primers were designed based on the sequence information of ferritin genes reported in the literature and used for rapid amplification of cDNA ends(RACE) for cloning Phascoloma esculenta ferritin gene.
根据已报道的铁结合蛋白基因的保守序列设计引物,用cDNA末端快速扩增(RACE)法,成功地从可口革囊星虫体液中获得铁结合蛋白基因的全长序列。
6) rapid amplification of cDNA end
cDNA末端快速扩增
1.
Methods: According to the partial sequences deposited in GenBank,the full-length cDNA was obtained using the techniques named rapid amplification of cDNA ends,and then,the sequence was analyzed bioinformatically.
方法:根据GenBank中已发表的NRDR的cDNA部分序列信息,运用cDNA末端快速扩增技术得到全长cDNA,然后利用生物信息学的方法对其进行分析。
补充资料：分子扩增
分子式：
CAS号：
性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。
CAS号：
性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条