1) RACE-PCR
迅速扩增cDNA末端PCR
1.
Full-length tree shrew PD-1 cDNA was cloned from spleen of tree shrew by rapid amplification of cDNA end-PCR(RACE-PCR).
利用迅速扩增cDNA末端PCR(RACE-PCR)技术,从树鼩脾组织中克隆了PD-1基因的全长cDNA序列。
2) random amplification of cDNA end PCR
cDNA末端快速扩增PCR
3) RACE
[英][reɪs] [美][res]
快速cDNA末端扩增
1.
The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).
利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
4) RACE
[英][reɪs] [美][res]
cDNA末端快速扩增
1.
METHODS: The full-length cDNA of rabbit BK_Ca beta subunit gene was amplified by 3′ RACE, 5′ RACE and RT-PCR from rabbit sphincter of Oddi (SO).
方法:采用3′及5′cDNA末端快速扩增(RACE)及RT-PCR技术从兔Oddi括约肌(SO)组织获得BKCa通道β亚基的全长cDNA序列。
2.
To find a novel gene which is related to protein of bovine seminal plasma (BSP) in human and study the new gene′s structural feature,expression and location in chromosome,the cDNA was cloned by using the 3′ rapid amplification of cDNA ends (RACE) and 5′ RACE methods and cloned into the expression vector of GST fusion protein (PGEX-5X-1),finally expressed in E.
采用cDNA末端快速扩增 (RACE)技术 ,克隆了一个BSP蛋白相关基因的cDNA序列 。
3.
Special primers were designed based on the sequence information of ferritin genes reported in the literature and used for rapid amplification of cDNA ends(RACE) for cloning Phascoloma esculenta ferritin gene.
根据已报道的铁结合蛋白基因的保守序列设计引物,用cDNA末端快速扩增(RACE)法,成功地从可口革囊星虫体液中获得铁结合蛋白基因的全长序列。
5) rapid amplification of cDNA end
cDNA末端快速扩增
1.
Methods: According to the partial sequences deposited in GenBank,the full-length cDNA was obtained using the techniques named rapid amplification of cDNA ends,and then,the sequence was analyzed bioinformatically.
方法:根据GenBank中已发表的NRDR的cDNA部分序列信息,运用cDNA末端快速扩增技术得到全长cDNA,然后利用生物信息学的方法对其进行分析。
6) rapid amplification of cDNA ends
cDNA末端快速扩增
1.
MATERIAL AND METHODS: Based on the rapid amplification of cDNA ends(RACE)method,gradient and nested PCR protocols were used to obtain full_length cDNA in order to get .
本研究应用cDNA末端快速扩增法(rapidamplificationofcDNAends,RACE)扩增此未知基因的全长,以进行下一步的基因功能研究。
补充资料：迅速
1.速度高，非常快。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条