1) Real-time fluorescent quantitative TRAP
实时荧光定量端粒重复序列扩增法
2) PCR-TRAP
端粒酶重复序列扩增法
1.
Methods Telomerase activity was measured by PCR-TRAP in cancerous lesions brushing cells from patients with lung cancer and lesions brushing cells from patients with noncancerous pulmonary disorders;CEA in bronchial washing fluid and in blood was measured by radioimmunoassay ,in which the specimens were obtained by fiberoptic bronc.
方法 采用端粒酶重复序列扩增法和放免法测定分别检测肺癌病变组织 (12 0例 )、非癌性肺疾病 (30例 )纤支毛刷刷脱细胞的端粒酶活性和毛刷涮洗液、血CEA浓度 ,并和病理学及细胞学检查结果进行比较。
3) TRAP
[英][træp] [美][træp]
端粒重复序列扩增法
1.
Methods The telomeric repeat amplification protocol (TRAP)and the silver staining assay were used to detect the telomerase activity in the tissue biopsy specimens and the brush biopsy specimens of bronchofiberscope from 35 patients with lung cancer and 35 patients with chronic bronchitis or benign pulmonary disease.
方法 采用以聚合酶链反应 (PCR)为基础的端粒重复序列扩增法 (TRAP) ,结合聚丙酰胺凝胶电泳银染法检测经纤维支气管镜取出的 3 5例肺癌患者肺癌组织和相应健侧肺支气管粘膜及 3 5例支气管 肺良性疾病患者支气管粘膜的端粒酶活性。
2.
Methods Telomerase activity was detected by a non-isotopic PCR-based TRAP (telomeric repeat amplication protocol) assay in tissue samples from 46 PGC,22 tumor-adjacent tissues and 19 benign lesions.
方法采用端粒重复序列扩增法(TRAP)检测了胆囊癌细胞系,46例原发性胆囊癌组织,22例癌旁组织,19例胆囊良性病变。
4) telomeric repeat amplification protocol enzyme linked immunosorbent assay
端粒重复序列扩增法酶联免疫吸附测定
5) telomerase repeat amplification protocol (TRAP)
端粒重复序列扩增技术
1.
Methods: Telomerase repeat amplification protocol (TRAP) assay was used to examine telomerase activity in forty-eight brain gliomas and eight normal brain tissues.
方法 :应用端粒重复序列扩增技术 (TRAP)对 4 8例胶质瘤标本和 8例正常脑组织的端粒酶活性进行检测。
6) Telomeric repeat amplification protocol
端粒重复序列扩增
1.
Telomerase activity was examined in 25 glioblastomas by telomeric repeat amplification protocol-silver staining.
[方法]采用端粒重复序列扩增 -银染法检查了25例胶质母细胞瘤的端粒酶活性。
补充资料：胶束增敏荧光法
分子式：
CAS号：
性质：荧光体系中引入表面活性剂，利用其胶束的增溶、增稳，特别是增敏作用而建立的荧光分析法。表面活性剂胶束为荧光物质提供的保护性微环境，减小了分子间碰撞失活和其他猝灭作用，使荧光强度提高，从而使测量灵敏度也显著提高。
CAS号：
性质：荧光体系中引入表面活性剂，利用其胶束的增溶、增稳，特别是增敏作用而建立的荧光分析法。表面活性剂胶束为荧光物质提供的保护性微环境，减小了分子间碰撞失活和其他猝灭作用，使荧光强度提高，从而使测量灵敏度也显著提高。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条