1) Western blot
免疫印迹杂交
1.
The allergic components were detected using Western blot with sera obtained from 48 house dust mite allergic subjects, and the IgE-binding capacity of the allergic components was analysed with the software BandScan5.
结果免疫印迹杂交检测到屋尘螨的10条致敏带,相对分子质量(Mr)依次为103、99、68、62、57、37、33、29、25、15×103,其中致敏带1、2、4、5、6、7、8的阳性反应率超过95%;致敏带10的阳性反应率为47。
2) Immunchistochemistry and Western blot
免疫组织化学和Western印迹杂交法
3) immunoblotting
免疫印迹
1.
An analysis and study of immunoblotting of different parts of protein of Fasciola hepatica;
肝片吸虫不同部位蛋白质的免疫印迹分析研究
2.
An analysis of 2-D electrophoresis and immunoblotting of 18.6kD protein antigen of Fasciola hepatica;
肝片吸虫18.6kD蛋白抗原双向电泳及免疫印迹分析
3.
Preparation of anti-human PD-1 antiserum with high-affinity and its application in PD-1 immunoblotting analysis;
抗人PD-1高亲和力抗血清的制备及其在PD-1免疫印迹分析中的应用
4) Western blot
免疫印迹
1.
Comparison on ELISA,Electroselenium method and Western blot for detection of anti-HIV antibody;
ELISA、胶体硒和免疫印迹试验检测HIV抗体结果分析
2.
Estimation of postmortem interval by determination of troponin I using Western blot technique in human pectoralis major;
免疫印迹检测人胸大肌肌钙蛋白I推断较晚期死后经过时间
3.
Diagnosis of Bovine Spongiform Encephalopathy and Scrapie by Western blot;
免疫印迹法检测牛海绵状脑病和羊瘙痒病
5) western-blot
免疫印迹
1.
Liver stage proteins were analyzed by Western-blot.
方法制备针对疟原虫唾液腺子孢子的免疫血清,通过免疫印迹识别肝细胞内疟原虫裂殖体的相关蛋白,并对识别的蛋白进行肽指纹图谱分析。
2.
Pure fimbriae were verified by Western-blot with monoclonal antibody (Mab) of F107.
用针对F107菌毛的单抗做免疫印迹反应,证实此条带为F107菌毛蛋白。
3.
pylori with coccoid variety by means of prolonged incubation and subinhibitory antibiotics, and then, studied the differences between the variety strain and its prototype through SDS-PAGE, Western-blot, furthermore, analysing the diferences of four virulent genes by PCR and PCR-SSCP respectively.
为研究幽门螺杆菌(HP)球形变异本质,作者通过延期培养和采用亚抑菌浓度抗生素,使3株HP发生球形变异,对弯曲形和球形HP作了SDS-PAGE、免疫印迹及4个毒力基因片段PCR和PCR-SSCP分析。
6) Immunoblot
['imjunəublɔt]
免疫印迹
1.
Development of Immunoblot Kit for the Detection of Anti,Toxoplasma Antibodies;
弓形虫抗体免疫印迹试剂盒的研制
2.
Analysis of immunoblot patterns of IgM and IgG antibodies in human sera of patients infected by Toxoplasma gondii;
弓形虫感染者血清IgM和IgG抗体免疫印迹谱分析
3.
Experimental research on protein immunoblot assay;
蛋白质免疫印迹技术的实验研究
补充资料:印迹杂交
分子式:
CAS号:
性质:在基因操作中,把DNA或RNA、蛋白质等在薄膜滤器上先经浸润,固定后,于薄膜滤器上进行杂交,生成杂种分子。为基因操作中最常用的技术。常用的操作方法是:将用凝胶电泳分离出来的DNA或RNA、蛋白质等,在保持电泳图形的条件下,使其移至薄膜滤器上,再用事先准备好的检测样品(探针,经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术,而把转移蛋白质称为韦斯顿(Western)印迹技术,当利用凝胶进行抗原抗体反应,再进行印迹时,称做伊斯特尔(Eastern)印迹技术。
CAS号:
性质:在基因操作中,把DNA或RNA、蛋白质等在薄膜滤器上先经浸润,固定后,于薄膜滤器上进行杂交,生成杂种分子。为基因操作中最常用的技术。常用的操作方法是:将用凝胶电泳分离出来的DNA或RNA、蛋白质等,在保持电泳图形的条件下,使其移至薄膜滤器上,再用事先准备好的检测样品(探针,经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术,而把转移蛋白质称为韦斯顿(Western)印迹技术,当利用凝胶进行抗原抗体反应,再进行印迹时,称做伊斯特尔(Eastern)印迹技术。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条