Methods of initiation RNA interference with siRNA expression cassettes in primary cells

Objective To compare the content and phenotype of cancer stem cells in the MCF-7,MDA-MB-231S human breast cancer cell line with those in the human breast cancer primary cells.

Objective: To approach the optimal concentration of trypsin and the best method on the dissociation of the tissue to obtain separated primary cells.

The effect of IL-10 on proliferation of primary cultured hepatocytes;

Rat primary cultured hepatocytes were isolated by a modified two-step collagenasedigestion method, the gradient concentration of Bacalin, Wogonin, Bacalin-Wogonin (1:1),BWEP treated the hepatocytes for 24 and .

But, the isolation and culture of primary hepatocytes in vitro is elaborate and the metabolic activity keeps too short, which causes the high cost and the failure in flexibility of hepatocyte application.

To investigate the sepraration method and morphological changes of long-term culture on primary adult chicken hepatocytes,the chicken primary hepatocytes were isolated by two-step in situ collagenase perfusion method,the culture condition was optimized,and the primary compouds of culture solutions were tried.

Objective To investigate the protection of tea polyphenol (TP) against the ethanol induced damage on the mice primary hepatocytes.