An experimental model in which alkylating agent N methyl N′ nitro N nitroguanidine (MNNG) with low concentration was employed to induce genetic instability of a monkey kidney vero cell line, it was proved that there were changes in the patterns of protein tyrosine residue phosphorylation and the activation of stress activated kinase(JNK/SAPK).

INFLUENCE OF MNNG ON PHOSPHATASE IN THE CULTURED CELLS FROM ADULT SCHISTOSOMA JAPONICUM;

To observe the effects of the bio-matrix from liver of rabbits (liver matrix) and N-methyl-N-nitro-N-nitrosoguanidine (MNNG) on the content and distribution of carbohydrates in the cultured cells from adult Schistosoma japonicum, the cultured cells from 32 days old S.

Methods By rectal perfusion,Wistar rats were given MNNG every day,and samples were collected periodically.

Cytotoxic effects of the main active components from three Chinese herbal medicines on N-methyl-N -nitro-N-nitrosoguanidine transformed GES-1 cells;

Methods:Hav- ing been treated by 200 mg/L PNS,the oncogene protein expression in N-methyl-N -nitro-N-nitrosoguanidine(MNNG)transformed human gastric epithelial cell line GES-1(MC cell)was detected by immunohistochemical technique and compared with that effected by retinoic acid as control.

Analysis of the transcription factor binding sites in the promoter region of genes encoding the co-expressive proteins induced by N-methyl-N -nitro-N-nitrosoguanidine;