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1)  real-time quantitative MSP
实时荧光定量甲基化特异性聚合酶链反应
2)  real-time reverse transcription polymerase chain reaction
实时荧光定量反转录聚合酶链反应
1.
Methods The expression of PBMC IP-10 mRNA in 42 children with KD and 40 healthy children were assayed by real-time reverse transcription polymerase chain reaction(real-time RT-PCR).
方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。
3)  real-time fluorescence reverse transcription polymerase chain reaction (RT-PC
荧光实时定量反转录-聚合酶链反应
4)  real-time polymerase chain reaction
实时荧光定量聚合酶链反应
1.
Methods DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis.
 方法 以实时荧光定量聚合酶链反应 (real- time PCR)方法检测 DNASE1的 m RNA表达水平 ,以毛细管电泳技术分析 m RNA编码区替代剪接体 ,结合单核苷酸多态性 (single nucleotide polymorphisms,SNPs)单倍型了解基因结构对表达的影响。
5)  real-time fluorescence relative quantitative RT-PCR
实时荧光定量-逆转录-聚合酶链反应
6)  real time quantitative RT-PCR
实时荧光定量逆转录聚合酶链反应
1.
Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of AngⅡwere observed at 3 hours,24 hours,3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively.
L-1O2)2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。
补充资料:精子特异性酶乳酸脱氢酶X(LDHX)的测定


精子特异性酶乳酸脱氢酶X(LDHX)的测定


  诊法。精液生化检查项目之一。测定方法主要用聚丙烯酰胺凝胶电泳,酶联染色及光密度计扫描法,可见6条区带,在LDH3与LDH4之间有一条LDHx区带,与生精能力有关。为精子细胞、精子和伴有精子的精液所有,与精子数量和酶的活性有关。可作为估价男子生育能力的客观指标。棉酚可特异性地抑制之,以影响生育。
  
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