1) Western blot
蛋白印迹
1.
Methods The immunohistochemistry SABC method and Western blot were used to determine the expression of Annexin A1 in 120 cases of normal breast tissues,ductal hyperplasia,non-invasive carcinoma and invasive carcinoma.
方法应用免疫组织化学SABC方法和蛋白印迹检测乳腺正常组织、良性病变(纤维腺瘤)、癌前病变(乳腺囊性增生症)、非浸润性导管癌、浸润性导管癌中Annexin A1的表达情况。
2.
The aliquot of proteins was subjected to SDS-PAGE,followed by Western blot analysis in which individual sera were tested for primary antibody.
用肺癌患者及健康对照者的血清作第一抗体进行蛋白印迹检测。
3.
Methods The immunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases(33 cases by IHC and 14 by Western Blot)of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues.
方法分别应用免疫组化方法(IHC)和蛋白印迹方法(Western Blot)检测47例鼻咽癌组织(其中用IHC33例,Western Blot14例)及24例鼻咽部炎症组织中COX-2蛋白的表达。
2) Western blotting
蛋白印迹
1.
METHODS:PDGFRαand C-kit gene mutations and protein expression in 52 cases of GIST were detected by polymerase chain reaction single- strand conformation polymorphism,immuno- histochemistry and Western blotting.
方法:采用单链象多态性聚合酶链式反应(PCR-SSCP),免疫组化和蛋白印迹(Western blot)方法,检测GIST 52例中PDGFRα和C-kit基因突变及蛋白表达情况。
2.
Method:Western blotting technique was used to test 60 serum samples.
方法 : 用蛋白印迹技术检测与麦芽糖结合蛋白 (MBP)融合的 2种重组梅毒螺旋体蛋白 (rTP47和rTP17)与二期梅毒患者血清的反应性。
3) Western-blot
蛋白印迹
1.
The levels of bcl-2 and bax expression in cardiomyocytes were measured by RT-PCR and Western-blot.
光镜下观察细胞形态;逆转录-多聚酶链反应和蛋白印迹法检测bc l-2、bax蛋白在心肌细胞中的表达情况。
4) Western blot
蛋白质印迹
1.
The expression of FATP4 genes in fatty liver rats was examined by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) and Western blotting.
方法 建立高脂饮食脂肪肝模型 ,用半定量逆转录聚合酶链反应 (reversetranslationpolymerasechainreaction ,RT PCR)与蛋白质印迹 (Westernblot)方法测定脂肪肝肝组织中FATP4表达变化。
5) Western blotting
蛋白质印迹
1.
The expression of phosphor-p42/44 MAPK was measured by Western blotting.
方法 :DADS处理CNE2细胞 2 4h后 ,荧光显微镜下观察形态学变化及凋亡细胞计数 ,四甲基偶氮唑盐微量酶反应比色法测定细胞活性 ,流式细胞仪检测凋亡细胞 ,蛋白质印迹法检测磷酸化p4 2 4 4MAPK表达。
2.
The expression of phospho p38 MAPK was measured by Western blotting.
方法 DADS处理CNE2细胞 2 4h后 ,荧光显微镜下观察形态学变化及凋亡细胞计数 ,MTT法测定细胞活性 ,流式细胞仪检测凋亡细胞 ,蛋白质印迹法检测磷酸化p38MAPK表达。
3.
Using Western blotting technique, the effects of auditory deprivation and tone exposure on the relative expression level (REL) of NMDA receptor subunit NR2B protein of rat auditory cortex during early postnatal days were studied.
应用蛋白质印迹检测技术,研究早期听觉剥夺、经验对大鼠听皮层NMDA受体NR2B蛋白表达的影响。
6) Western blotting
蛋白印迹法
1.
Radioimmunoassay, RT-PCR and Western blotting techniques were used to evaluate the change of brain COX expression at different time points( before SNI, 1 h, 12 h, 1 d, 3 d, 7 d, 14 d, 30 d and 60 d after SNI); By exploring hot plate test, we observed the reacting time of animals after injection of saline, NS-398, SC-560 and ind.
对小鼠施行SNI后,我们采取放射免疫法,RT-PCR法和蛋白印迹法测定术前以及术后1h、12h、1d、3d、7d、14d、30d、60d等不同时间点的脑COX-1和COX-2的表达;采用热板法,分别测定小鼠在各时间点注射生理盐水、NS-398、SC-560或吲哚美辛后的疼痛阈值。
2.
Methods: By using Western blotting (WB), RPR test and TPPA, serologic antibodies were detected in 67 cases of syphilis, of whom 21 were followed up for 18 to 24 months after treatment.
方法:采用蛋白印迹法(Westernblotting,WB)、快速血浆反应素(RPR)试验、梅毒颗粒凝集试验(TPPA)3种方法,对67例梅毒及其中经治疗后随诊18～24个月的21例患者作血清检测。
3.
The techniques of diagnosis include ELISA and Western blotting.
细菌病原诊断技术包括酶联免疫吸附技术（ＥＬＩＳＡ）及蛋白印迹法（Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ）。
补充资料：蛋白印迹法
分子式：
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
CAS号：
性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条