1) overlap extension
重叠延伸
1.
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
2) Overlap-extension
重叠延伸
1.
Amplification of DNA sequence Encoding N-terminal Fragment of Osteoprotegerin Using Overlap-extension PCR;
重叠延伸PCR法在合成人骨保护素N端编码序列中的应用
3) signal peptide
重叠延伸
1.
CAD, then using SOE(splicing by overlap extension) technique to fuse CD137 signal peptide gene into LIGHT extramembrane domain encoding sequence, inserting this recombinated LIGHT cDNA with human IgG1 Fc cDNA into the eukaryotic expression plasmid pcDNA3, .
测序证实后 ,继续用PCR扩增其膜外区cDNA ,用重叠延伸技术(SOE)引入CD137信号肽基因。
4) Overlap extension PCR
重叠延伸PCR
1.
1 as the template,the PSD-95 mutant genes were amplified by overlap extension PCR,and then were cloned into the plasmids of PSD-95-pcDNA3.
1为模板,采用重叠延伸PCR法获得突变型PSD-95的局部或全长cDNA片段,并克隆至PSD-95-pcDNA3。
2.
According to the amino acids sequence of OC-IΔD86 gene and Escherichia coli codon usage, we synthesized this gene by overlap extension PCR method with 7 oligonucleotides DNA fragments.
根据OC-IΔD86基因序列,设计合成了7条寡核苷酸片段,通过重叠延伸PCR技术合成了OC-IΔD86基因,利用设计好的BamH I/Xho I酶切位点将OC-IΔD86基因克隆到原核表达载体pet21b中,在1mmol/L的IPTG诱导后5h,OC-I?D86融合基因在大肠杆菌中得到表达,表达产物处于可溶状态,其表达量占总蛋白的11。
3.
How to create two separate site-specific mutagenesis in a DNA fragment using overlap extension PCR was explored.
探讨如何利用重叠延伸PCR对同一靶DNA片段中的两个不同位点实施联合突变。
5) overlapping extension PCR
重叠延伸PCR
1.
Cell culture-adaptive mutations were carried out by the method of overlapping extension PCR OE-PCR at the sites of E1202G T1280I and S2197P in the genes of ns3 and ns5a.
方法:根据HCV全长基因组cDNA序列及突变位点设计引物,运用重叠延伸PCR法对ns3和ns5a基因的E1202G、T1280I和S2197P位点进行细胞培养适应性突变,将突变后的片段分别克隆入pBluescriptIIKS(+)、pRSET-A载体,经测序正确后,分别连入含有HCV全长cDNA的质粒的H/FL相应位置,置换原未突变片段,并进行PCR及酶切鉴定。
6) overlap extention PCR
重叠延伸PCR
1.
K88ac-STⅡ fused gene amplified by overlap extention PCR was cloned into T vector.
利用重叠延伸PCR技术将K88ac STⅡ融合基因克隆于T载体上,将重组基因质粒转化到受体菌DH10B中,蓝白斑筛选阳性菌落,通过PCR、NcoI/XcoI酶切后测序,与genbank报道的K88ac结构基因序列进行比对,证明所克隆的目的片段为K88ac STII融合基因。
补充资料：多肽链延伸因子
多肽链延伸因子
多肽链延伸因子 polypeptide chain elonga－tion factor
蛋白质生物合成中参与多肽链延伸过程的蛋白质因子。从大肠杆菌等原核细胞可纯化获得三种延伸因子（elongation factor），即eftu，efts和efg，分子量大约分别为4.7万，3.6万和8.3万。eftu与gtp结合成eftu-gtp，然后再与氨酰trna结合，形成三复合体（ternary complex）氨酰-trna-eftu－gtp。这种三复合体与核糖体的氨酰trna部位（a部位，aminoacyl site）结合，继而gtp被分解，eftu以eftu－gtp的形式从核糖体上游离出来。游离的eftu－gdp与efts反应，再生成eftu－ts，然后与gtp结合成eftu－gtp。另一方面结合在p部位（peptide transfer）。多肽链即可延长一个氨基酸残基。接着肽基trna从a部位转移（translccation）到p部位，p部位的trna从核糖体上脱离下来。该反应由efg（g因子，亦称为移位酶）催化，再将一分子的gtp水解。如图所示，由于上述反应的逐次反复进行，而使多肽链延伸反应得以进行，每延长一个氨基酸残基，就水解两分子gtp。动物细胞或其他真核细胞基本上也以同样机制进行多肽链的延伸，并且也分离到了对应于原核细胞的各因子。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。