1) splicing by overlapping extens
重叠延伸剪接术
1.
Methods The authors used the method of splicing by overlapping extension (SOE) to obtain the competitive DNA fragment, with which they set up the competitive quantitative RT PCR assay to detect AML1 ETO chimeric genes in the patients of t(8;21) AML.
方法 采用重叠延伸剪接术 (splicing by overlapping extention,SOE)获得竞争性 DNA片段 ,建立竞争性定量 PCR方法 ,并应用于 t(8;2 1) AML 患者的检测。
2) splicing by overlapping extension(SOE)
重叠延伸剪接技术
3) SOE
重叠延伸剪接法
1.
Methods Splicing by overlap extension(SOE) was used for deletion of HIV 1 ADA V3 loop.
方法分别设计包膜糖蛋白两端及V3环两端的两对引物,采用重叠延伸剪接法,进行HIV1ADA株包膜糖蛋白V3环缺失体的构建。
4) splicing by overlapping extention
重叠延伸拼接
5) splicing by overlap extension
PCR重叠延伸技术
1.
Methods The PCR product of signal and mature peptides of HD-5 were linked by splicing by overlap extension(SOE).
方法通过PCR重叠延伸技术得到HD-5信号肽及成熟肽的嵌合分子,并将其插入真核表达质粒pVITRO3。
2.
The PCR product of signal and mature peptides of GLP2 was linked by SOE (splicing by overlap extension) and the gene, which coded the second amino acid in the N-terminal of GLP2, was mutated from GCT to GGT by PCR.
方法通过RT-PCR方法获得含GLP2编码序列的高血糖素原cDNA片段;通过PCR重叠延伸技术得到GLP2信号肽及成熟肽的嵌合分子,并将编码GLP2成熟肽N-末端第二位丙氨酸的序列(GCT)突变为编码甘氨酸的序列(GGT);将其插入真核表达质粒pVITRO3,转染至肠上皮细胞(Caco-2),观察其对细胞增殖活性的影响。
6) splicing by overlap extension (SOE)
重叠延伸技术(SOE)
补充资料：术术
1.兴盛貌。 2.宽广貌。
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