1) Pichia pastoris
毕赤酵母细胞
1.
Expression,purification and identification of recombinant sCR1 in Pichia pastoris;
重组sCR1在毕赤酵母细胞中的表达、纯化及鉴定
2.
The full length cDNA of human sCR1 gene was obtained by RT-PCR and them, cloned into Pichia pastoris eukaryotic expression vector pPIC9k to construct the recombinant plasmid pPIC9k-sCR1 containing human sCR1.
方法:从人外周血中提取总RNA,应用RT-PCR获得人sCR1全长cDNA,然后将其克隆入毕赤酵母细胞分泌型表达载体pPIC9k中,构建含人sCR1的重组质粒(pPIC9k-sCR1),经测序鉴定正确,电转化入毕赤酵母细胞SMD1168中,将经G418抗性筛选出的重组sCR1酵母细胞株进行PCR鉴定,经甲醇诱导,表达产物经SDS-PAGE分析和Western blot鉴定,通过Ni2+-NTA agarose亲和层析纯化后进行生物学活性鉴定。
3.
High Level Expression of Human Phospholipid Transfer Protein in the Yeast Pichia Pastoris;
为深入了解磷脂转运蛋白的结构和功能 ,从中国人胎肝组织总RNA成功克隆了磷脂转运蛋白成熟肽基因 ,所得磷脂转运蛋白基因通过同源重组整合至毕赤酵母细胞染色体的醇氧化酶AOX1基因中 ,甲醇诱导后经SDS聚丙烯酰胺凝胶电泳显示诱导表达蛋白分子量约 75kDa,薄层扫描显示表达蛋白占酵母蛋白总量的 2 8% ,为大量制备重组磷脂转运蛋白奠定了基
2) Pichia pastoris
毕赤酵母
1.
Feed-batch fermentation of Pichia pastoris engineering strain;
毕赤酵母工程菌产植酸酶补料分批发酵研究
2.
Effects of glycerol feeding strategies on production of recombinant reteplase from a methylotrophic yeast Pichia pastoris;
甘油相控制策略对重组毕赤酵母表达瑞替普酶(reteplase)的影响
3.
Artificial Neural Network-based Modeling of Biomass Concentration of Recombinant Yeast Pichia Pastoris at Expression Phase;
基于人工神经网络的重组毕赤酵母表达期菌体浓度模型
3) P.pastoris
毕赤酵母
1.
Expression of Soluble Recombinant Human Antithrombin Ⅲ(AT-Ⅲ) inP.pastoris;
人抗凝血酶Ⅲ(AT-Ⅲ)在毕赤酵母中可溶性表达
2.
Study on the transduction activity of Tat oligopeptide (I)——expression and purification of the model protein Tat-GFP in P.pastoris;
Tat短肽跨膜递送作用的研究(I)——模型蛋白Tat-GFP在毕赤酵母中的表达与纯化
3.
High Level Secretion Expression of Beefy Meaty Peptide Gene in P.pastoris;
牛肉风味强化肽(BMP)在毕赤酵母中的高效分泌表达
4) Pichia pastoris yeast
毕赤酵母
1.
Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells;
抗菌肽D基因在毕赤酵母中的表达及鉴定
2.
The extended DNA was assembled onto Pichia pastoris yeast X33 for expression.
设计并克隆了一个以源于蚕丝丝素蛋白结晶区的序列为GAGAGS(GlyAlaGlyAlaGlySer)的典型肽段为基本框架的基因,并导入毕赤酵母(Pichiapastoris)X33内进行了表达。
5) Pichia
毕赤酵母
1.
Expression in Pichia pastoris, fermentation and purification of HIV-1 CN54 Gag antigen;
HIV-1中国株CN54 Gag蛋白在毕赤酵母中的表达纯化和鉴定
2.
Expression and selection of single-chain Fv-Fc(scFv) antibody fragment against antigen of rabies virus in Pichia pastoris;
应用毕赤酵母分泌表达筛选人抗狂犬病毒抗体scFv-Fc
3.
Construction of human antimicrobial peptide LL-37 vector and its expression in Pichia pastoris;
人源抗菌肽LL-37表达载体的构建及其在毕赤酵母中的表达
6) pichia pastories
毕赤酵母
1.
Sequence analysis of human papillomavirus 16 L1 gene from Guangdong area and construction of its pichia pastories expression vector;
广东地区HPV16型L1基因的序列分析及其毕赤酵母表达载体的构建
2.
Secretive expression of Mycobacterium tuberculosis MPT64 in Pichia pastories;
结核分枝杆菌MPT64在毕赤酵母中的分泌表达
3.
Objective To construct the Pichia pastories secretion type expression vector of human soluble BLyS mutants,which paved a way for further investigation on the function and mechanism of sBLyS.
目的构建人BLyS胞外区(soluble BLyS)突变体毕赤酵母分泌型表达载体,为深入探讨BLyS的功能和机制创造条件。
补充资料：赤后
1.指南方。古以南方配火，其色赤;后即后土，土地。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条