1) RT-PCR
反转录聚合酶链反应
1.
[Methods] After rats of groups A and B were administrated by gavage with liquid of Epimedium and Fructus Ligustri Lucidi for 14 days respectively, group C as the control, RT-PCR technique was used for examining mRNA expression and immunohistochemistry was used for examining protein exp.
方法SD雄性大鼠,分为A、B、C3组,A、B分别给予补肾阳中药淫羊藿和补肾阴中药女贞子持续灌胃14d,C组作为空白对照,采用反转录聚合酶链反应(RT-PCR)方法检测雌激素受体ERβmRNA表达量,免疫组化染色检测ERβ蛋白的分布及表达。
2.
Total RNA were abstracted from retinal and choroidal tissue and the mRNA of PEDF was detected by RT-PCR.
采用半定量反转录聚合酶链反应(RT-PCR)方法检测PEDF mRNA在大鼠视网膜中的表达水平的变化。
3.
Methods HUVECs were incubated with different concentrations of LHS and the TF mRNA expression was detected by reverse transcript-polymerase chain reaction (RT-PCR).
测定不同浓度LHS处理前后组织因子表达,应用反转录聚合酶链反应(RT-PCR)法检测TFmRNA水平。
2) Reverse transcriptase polymerase chain reaction
反转录聚合酶链反应
1.
Methods The c-myc cDNA was acquired by reverse transcriptase polymerase chain reaction(RT-PCR) after isolating total RNA from tissue of human esophageal squamous cell carcinoma(ESCC).
方法用反转录聚合酶链反应(RT-PCR)法从人食管鳞癌组织的总mRNA中反转录扩增c-myc cDNA,将纯化的PCR产物与pGEM-T Easy载体进行连接,转化宿主菌DH-5α,然后挑选阳性克隆重组质粒,分别用PCR扩增、限制性核酸内切酶EcoRⅠ酶切进行鉴定并测序分析。
3) reverse transcription polymerase chain reaction
反转录聚合酶链反应
1.
METHODS: The expression levels of mRNA and protein of survivin were investigated respectively by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods in cell lines BT325 and SHG-44.
方法 :采用反转录聚合酶链反应 (RT PCR)和免疫组化方法分别检测SurvivinmRNA及蛋白在BT32 5和SHG 4 4细胞系中的表达水平 。
4) reverse transcription-polymerase chain reaction
反转录聚合酶链反应
5) reverse transcriptase-polymerase chain reaction
反转录聚合酶链反应
1.
Methods Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.
方法在2003年秋冬季腹泻流行期间在南方医院儿科收集临床诊断为病毒性腹泻患儿的粪便标本,并采用半套式反转录聚合酶链反应(RT-PCR)方法检测。
6) RT-PCR
反转录-聚合酶链反应
1.
Studies on the Detection of Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR;
用反转录-聚合酶链反应检测猪繁殖与呼吸综合征病毒的研究
2.
The expression of CGRP mRNA in trigeminal ganglion were detected with reverse transcription-polymerasa chain reaction(RT-PCR).
方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节CGRP mRNA水平的变化。
3.
Methods The mRNA expression of the survivin gene was evaluated by reverse transcription-polymerase chain reaction(RT-PCR) in 40 laryngeal carcinomas and in 52 non-neoplastic laryngeal tissues.
方法应用反转录-聚合酶链反应(RT-PCR)检测喉鳞癌组织、癌旁组织、非典型增生组织和正常对照组织中Survivin mRNA的表达水平。
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条