1) RT-PCR
逆转录-聚合酶链反应
1.
Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RASSF1A mRNA in 29 cases of human transitional cell carcinoma of bladder and 5 cases of normal bladder tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)检测29例膀胱移行细胞癌组织及5例正常膀胱组织中RASSF1A mRNA的表达水平。
2.
Results:RT-PCR and the sequence analysis of its product suggested that these 58 strains of measles viruses should belong to the H1 genotype of the eighth genetic group of wild measles virus.
结果:该58株病毒经逆转录-聚合酶链反应和其产物的基因序列分析显示,这58株病毒属于麻疹野病毒第8基因组H1基因型。
3.
Methords: The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinorma tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测45例PCa组织、30例前列腺高分级上皮样内瘤样病变组织(HG-PIN)、43例BPH组织和39例其他肿瘤组织标本中PCA-1 mRNA的表达。
2) Reverse transcription-polymerase chain reaction
逆转录-聚合酶链反应
1.
Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.
方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况。
2.
Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GRα and β mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.
方法采用逆转录-聚合酶链反应(RT-PCR)方法对18例GC治疗敏感、10例GC治疗抵抗的PM/DM患者PBMC中GRα、β mRNA的表达水平进行了检测,同时采用放射免疫分析法测定血清中的皮质醇含量,并与20例正常对照进行比较。
3.
Methods Using reverse transcription-polymerase chain reaction(RT-PCR),the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
3) Reverse transcription polymerase chain reaction
逆转录-聚合酶链反应
1.
Methods: The expression of p14ARF, ARF-BP1 and c-myc mRNAs was detected by reverse transcription polymerase chain reaction (RT-PCR) technique in the 45 HCC tissues and their non-tumorous liver tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)技术、免疫组化技术对45例HCC组织及其癌旁肝组织中p14ARF、ARF-BP1、c-myc的mRNA表达和p14ARF、c-myc蛋白表达情况进行检测。
2.
Methods: Reverse transcription polymerase chain reaction(RT-PCR) and streptavidin-peroxidase immunohistochemical method(S-P) were employed to measure the expression of aromatase cytochrome P450(CYP19) mRNA and P450 arom protein in rat ovary.
方法应用半定量逆转录-聚合酶链反应(RT-PCR)检测PCOS实验组及正常对照组大鼠卵巢组织中P450 arom基因(CYP19)的表达;采用链霉素抗生物素蛋白-过氧化物酶(SP)法对大鼠卵巢组织中P450 arom进行检测。
3.
Reverse transcription polymerase chain reaction(RT-PCR)is a main method to detect HMPV,and real-time qRT-PCR is becoming more and more popular for detecting it.
逆转录-聚合酶链反应(RT-PCR)技术是检测HMPV的主要手段,且荧光定量RT-PCR技术已经成为当前研究的常用方法。
4) Reverse transcriptase polymerase chain reaction
逆转录-聚合酶链反应
1.
hy926 cell as the positive controls and human embryonic lung WI38 as the negative controls,the expression of EPCR in 6 lung cancer cell lines were measured by FCM,Western blot and semi-quantitative reverse transcriptase polymerase chain reaction.
hy926内皮细胞作阳性对照,人胚肺WI38细胞作阴性对照,通过流式细胞术、West-ern blot及半定量逆转录-聚合酶链反应分析EPCR在6种肺癌细胞系的表达水平。
2.
NUP98-TOP1 transcript resulted from t(11;20) was assayed by reverse transcriptase polymerase chain reaction (RT-P.
目的 :报道 1例伴有t(11;2 0 ) (p15 ;q11)易位的急性单核细胞白血病 (AML -M 5 )病例及其染色体涂染、逆转录-聚合酶链反应 (RT -PCR)的研究结果。
3.
Methods The expression of B-FABP genes in normal kidney tissues(n=20) and surgically resected RCC tissues(n=55) was evaluated by reverse transcriptase polymerase chain reaction.
方法应用逆转录-聚合酶链反应(RT-PCR)检测55例RCC患者的癌组织、20例正常肾组织、12例健康人及47例RCC患者尿液中B-FABP mRNA的表达。
5) reverse transcriptase-polymerase chain reaction
逆转录-聚合酶链反应
1.
Methods The expression of DNMT1 mRNA was detected by semi-quantitative Reverse Transcriptase-Polymerase Chain Reaction(RT-PCR)in the cancer tissues and corresponding adjacent normal tissues in 34 gastric cancer patients.
方法采用半定量逆转录-聚合酶链反应(RT-PCR)检测34例原发性胃癌患者癌组织及癌旁正常组织中DNMT1 mRNA的表达,采用免疫组级化学方法检测DNMT1蛋白的表达情况。
2.
The expressions of procollagen typeⅠand type Ⅲ mRNA were measured by reverse transcriptase-polymerase chain reaction(RT-PCR).
分别于第6周末、第8周末取肝组织观察病理组织学变化;逆转录-聚合酶链反应(RT-PCR)检测Ⅰ、Ⅲ型前胶原(Ⅰ-PC、Ⅲ-PC)mRNA的表达。
6) Reverse transcription polymerase chain reaction
逆转录酶聚合酶链反应
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条