1) Western blot
Western印迹分析
1.
HSP72 mRNA and HSP70 expression in heart and vessel tissues of both simulated weightless and control rats exposed to heat stress (ambient temperature, Ta=43℃) and recovered at Ta of 25℃ for 1 h (CON H 1, SUS H 1) or 2 h (CON H 2, SUS H 2) were analyzed using Northern blot and Western blot.
用Northern杂交与Western印迹分析检测 4周模拟失重大鼠热应激后并在室温下恢复 1h (SUS H1)或 2h (SUS H2 )心肌、血管组织HSP70表达的变化。
2) Western blot analysis
Western印迹分析
1.
Western blot analysis showed the exogenous OsGSTL1 was expressed in transformed yeast.
Western印迹分析表明外源OsGSTL1基因在转基因酵母中表达,分析半乳糖诱导表达的酵母粗提液的谷胱甘肽转移酶活性表明:转基因酵母的谷胱甘肽转移酶较非转基因酵母和未诱导的酵母高,说明OsGSTL1在转基因酵母中受半乳糖的诱导表达,具有谷胱甘肽转移酶活性。
3) Western blotting
Western印迹分析
1.
The protein expression of AGT and AT 1 in basilar, carotid, femoral, and mesenteric arterial tissues were demonstrated by Western blotting and normalized by β actin for quantitat.
用Western印迹分析比较 4周模拟失重 (SUS)组与同步对照 (CON)组大鼠基底动脉、颈总动脉、股动脉和肠系膜动脉组织AGT及AT1 的蛋白表达变化。
4) Western dot assay
Western斑点印迹分析
5) Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
6) Western blotting
Western印迹
1.
METHODS: The mRNA level and protein expression of ANXA1 in some OSCC cell lines and human primary OSCC tissue specimens were detected using real-time PCR ,Western blotting and immunohistochemistry.
方法:应用实时定量PCR、Western印迹方法和免疫组化方法检测ANXA1在口腔鳞癌细胞株和30例原发口腔鳞癌中的表达,采用SPSS10。
2.
Methods The ES antigens (ESA) of Trichinella spiralis muscle larvae cultured in vitro at 18 h and 30 h were analyzed by SDS-PAGE and Western blotting.
方法 应用SDS PAGE和Western印迹对旋毛虫肌幼虫体外培养 18、3 0h后的ES抗原中的蛋白组分进行研究。
3.
9 breast cancer cell lines were cultured and cells harvested, protein of p50, p65 and IkB extracted from cytoplasm, nuclear separately to detect the expression of p50, p65, IkB proteins by Western blotting.
用 L-1 5培养液培养并传代 9个乳腺癌细胞系 ,收获细胞 ,提取胞浆、胞核蛋白质 ,Western印迹法观察 NFk B家族蛋白及其抑制物的表达 ;采用 2 0 J· m- 2 剂量紫外线照射细胞 ,继续培养细胞并提取蛋白质进行检测。
补充资料:Western blotting
分子式:
CAS号:
性质:一种类似于DNA斑迹法,用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
CAS号:
性质:一种类似于DNA斑迹法,用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条