1) nested inverse PCR
巢式反向PCR
2) Inverse nestd PCR
反向嵌套巢式PCR
3) RT-nested PCR
反转录巢式PCR
4) nested PCR
巢式PCR
1.
Amplify of fetal SRY gene in maternal plasma by using nested PCR;
应用巢式PCR扩增孕妇血浆中胎儿SRY基因
2.
Cloning of N-terminal deleted IκBα gene by nested PCR;
应用巢式PCR法克隆N端部分缺失的IκBα基因
3.
Establishment of a nested PCR assay for detection of Mycoplasma ovis;
羊附红细胞体巢式PCR检测方法的建立及临床应用
5) Nested RT-PCR
巢式RT-PCR
1.
Development of nested RT-PCR for detection animal rabies virus
动物狂犬病病毒巢式RT-PCR检测方法的建立
2.
To study the incidence, the types of fusion genes and the clinical significance of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), the rearrangements of MLL gene of 60 patients with AL were detected by fluorescence in situ hybridization (FISH) and 6 types of common fusion genes resulting from the rearrangements of MLL gene were detected by nested RT-PCR.
为了研究混合系白血病(MLL)基因重排在急性白血病(AL)中的发生率、融合基因类型及其临床意义,用荧光原位杂交技术检测60例急性白血病(AL)患者MLL基因重排,对于MLL基因重排阳性的患者,用巢式RT-PCR方法检测MLL基因重排形成的6种常见融合基因类型。
3.
Methods CK19mRNA expression was detected by nested RT-PCR in the peripheral blood from 60 lung cancer patients.
方法以CK-19为标记,采用巢式RT-PCR和半定量方法对肺癌患者外周血中微转移灶的检测,比较治疗前后外周血中癌细胞数的相对变化。
6) Nest PCR
巢式PCR
1.
A new method for identifing the change of single nucleotide by created restriction site PCR and Nest PCR;
联合应用巢式PCR和创造酶切位点法检测单核苷酸改变
2.
Utilization of Nest PCR in Intestinal Infection Caused by F subgenus of Enteric Adenovirus
巢式PCR在腺病毒F亚属引起的致腹泻肠道感染检测中的应用
3.
Methods The expressive levels of α1 integrin in specimens were determined by FQ-PCR technique and nest PCR.
方法应用荧光定量PCR和巢式PCR技术检测10例增生性瘢痕组织中整合素α1的表达,并以10例正常皮肤组织作为对照。
补充资料：反向PCR
分子式：
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条