1) Anion exchange chromatography
阴离子交换层析
1.
After breaking cells by ultrasonic and separating roughly CK20 by extraction, centrifugation, and dialysis, DEAE-cellulose anion exchange chromatography was used for further purification.
方法 培养人膀胱肿瘤T 2 4细胞系并用免疫组化检测CK2 0表达 ,然后大量培养并收集细胞 ,超声波细胞粉碎后 ,先用萃取、高速离心、透析等方式进行蛋白初分离 ,然后利用以DE5 2为介质的阴离子交换层析分离纯化CK2 0蛋白 ,以电泳示踪 ,最后采用SDS PAGE、HLPC和Western blotting加以鉴定。
2.
The enzyme was purified through three steps of anion exchange chromatography including DEAE-Sepharose,Source 15Q and Mono Q.
通过DEAE-Sepharose,Source15Q,Mono Q三步阴离子交换层析从细胞中得到纯酶,收率达40。
2) anion-exchange chromatography
阴离子交换层析
1.
To obtain the main peanut allergen Ara h2, defatted process, centrifugation, anion-exchange chromatography and membrane dialysis were used during the purification with the crude ground peanut as material.
结果显示,采用阴离子交换层析方法,制取的Arah2蛋白纯度达90%,得率为21。
2.
Alcaligenes faecalis penicillin G acylase(AfPGA) was isolated from recombinant Escherichia coli DH5α,and purified by a combination of DEAE-Sepharose anion-exchange chromatography and(Butyl)-Sepharose CL 4B hydrophobic chromatography.
采用DEAE-Sepharose阴离子交换层析和Butyl-Sepharose CL 4B疏水层析,从重组大肠杆菌DH5α菌体中分离纯化得到粪产碱杆菌青霉素G酰化酶(AfPGA)。
3) Anion exchange liquid chromatography
阴离子交换层析法
4) Weak anion exchange chromatography
弱阴离子交换层析
5) ion-exchange chromatography
离子交换层析
1.
Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography.;
离子交换层析复性重组人γ-干扰素折叠二聚体的形成
2.
Acetylcholinesterase(AChE) of Bombyx mori Linaeus,separated from the crude extract,was purified to electrophoretic homogeneity by Sephadex G-25 chromatography,DEAE-Sepharose Fast Flow ion-exchange chromatography and Sephacryl S-200 gel filtration,respectively.
采用了Sephadex G-25层析,DEAE-Sepharose Fast Flow离子交换层析和Sephacryl S-200凝胶过滤层析,对家蚕头部粗酶匀浆液中的乙酰胆碱酯酶进行纯化,得到的样品经聚丙烯酰胺凝胶电泳(PAGE)和SDS-PAGE检测均为一条带,用SDS-PAGE法测得其分子量为77。
3.
Then,BMP was isolated and purified by the methods of D301 ion-exchange chromatography(IEC)and Sephadex G-25 gel-filtration chromatography(GFC).
使用反相高效液相色谱(RP-HPLC)对重组酵母发酵液中一种牛肉风味肽(BMP)的稳定性进行了研究,并分别运用D301离子交换层析和Sephadex G-25凝胶过滤层析对其进行了分离纯化,最后利用RP-HPLC对目标肽进行分析检测。
6) DEAE iron-exchanged chromatography
DEAE离子交换层析
补充资料：离子交换树脂A101D 强碱性季铵I型阴离子交换树脂(A201型) 611强碱性季铵I型阴离子交换树脂
CAS：39421-71-1
中文名称：离子交换树脂A101D 强碱性季铵I型阴离子交换树脂(A201型) 611强碱性季铵I型阴离子交换树脂
英文名称：Duolite A101D Anion exchange resin, strong basic quarternary ammonium(I) A201； Anion exchange resin,strong basic quarternary ammonium(I)A201
中文名称：离子交换树脂A101D 强碱性季铵I型阴离子交换树脂(A201型) 611强碱性季铵I型阴离子交换树脂
英文名称：Duolite A101D Anion exchange resin, strong basic quarternary ammonium(I) A201； Anion exchange resin,strong basic quarternary ammonium(I)A201
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条