1) Homologous recombination
同源重组
1.
Construction of cDNA library from mouse oocyte for yeast-2-hybrid system by homologous recombination;
用同源重组法构建小鼠卵母细胞cDNA酵母双杂交文库的探索
2.
Construction of CHO high expression cell line by means of homologous recombination;
利用同源重组建立CHO高表达细胞株
3.
Construction of recombinant adeno-viral plasmid bearing hSDF-1α cDNA by homologous recombination in bac-teria and preparation of recombinant adenovirus expressing hSDF-1α;
细菌内同源重组快速构建和制备表达hSDF-1α的重组腺病毒
2) homogenous recombination
同源重组
1.
Then pDC316-BMP-7 and the framework plasmid pBHGlox_E1,3Cre were transfected into HEK293 cells for homogenous recombination in cells with Lipofectamine 2000.
方法用基因工程技术将人BMP-7基因cDNA亚克隆至穿梭质粒pDC316上,利用脂质体介导的方法将AdMax腺病毒包装系统的骨架质粒pBHGlox_E1,3Cre和穿梭质粒pDC316-BMP-7转染入HEK293细胞,进行同源重组,得到腺病毒重组质粒Ad5-BMP-7,并在其中包装扩增病毒。
2.
Methods The human HIF-1α gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria, and then was transfected into HEK293 cells with Lipofectamine TM 2000.
方法采用基因工程技术,经过亚克隆将人HIF-1α基因片段克隆至穿梭质粒pAdTrack-CMV上,利用pAdEasy系统进行细菌内同源重组后,经脂质体转染HEK293细胞,进行重组腺病毒的包装、扩增。
3.
Methods The human p16 gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria.
方法用基因工程技术将人p16基因cDNA亚克隆至穿梭质粒pAdTrack-CMV上,利用PAdEasy系统进行细菌内同源重组,然后通过脂质体将正确重组体包裹并转染293T细胞,并在其中包装扩增病毒。
4) DNA homologous recombination
DNA同源重组
5) Red recombination
Red同源重组
1.
The Escherichia coli strain SQ88 was selected as the target strain,to which five of rRNA operons(rrnA,rrnD,rrnE,rrnG and rrnH)were sequentially inactivated using λ Red recombination system.
以1株染色体上的5个rRNA操纵元(rrnA、rrnD、rrnE、rrnG和rrnH)被敲除的大肠杆菌(Escherichia coli)菌株SQ88为出发菌,运用λRed同源重组系统和卡那霉素抗性基因筛选重组子,并利用抗性基因两端的FRT位点通过位点专一性重组将抗性基因去除,最终成功构建了1株仅具有单拷贝rRNA操纵元(rrnB)的E。
6) λ Red recombination system
λRed同源重组
补充资料：同源重组技术
分子式：
CAS号：
性质：见基因打靶
CAS号：
性质：见基因打靶
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条