1) homologous recombination in bacteria
细菌内同源重组
1.
Objective To construct recombinant adenovirus by homologous recombination in bacteria.
目的通过细菌内同源重组方法构建重组腺病毒。
2.
While traditional AdEasy system is somehow complicated, less successful and may have special request on laboratory condition, the modified AdEasy system in which AdEasier-1 cells are constructed before homologous recombination in bacteria may simplify the procedure, shorten period of experiment, improve rate of success, lower request of condition and thus be used in more laboratory.
目的 建立一种改进的AdEasy系统应用于复制缺陷型腺病毒的构建,针对传统的细菌内同源重组法制备腺病毒过程仍较复杂,成功率低和对实验室条件要求高等缺点,通过先构建AdEasier-1细菌后进行细菌内同源重组来简化实验过程,缩短实验周期,提高成功率,降低对实验室条件的要求,以利于在更多实验室应用。
2) bacterial homologous recombination system
细菌同源重组系统
3) somatic homologous recombination
体细胞同源重组
1.
The somatic homologous recombination was frequently used to evaluate genome stability because it can result in DNA changes, such as rearrangement, deletion and duplication.
体细胞同源重组产生的DNA重排、缺失和复制等是基因组不稳定的重要指标,以拟南芥菜GUS基因重组报告系R2L100和R3L66为实验材料,以体细胞同源重组频率(每个植株上的GUS斑点数目)作为评估标准,研究低能Ar+离子和α粒子辐射对植物基因组稳定性的影响。
4) in vivo homologous recombination
体内同源重组
6) Homologous recombination
同源重组
1.
Construction of cDNA library from mouse oocyte for yeast-2-hybrid system by homologous recombination;
用同源重组法构建小鼠卵母细胞cDNA酵母双杂交文库的探索
2.
Construction of CHO high expression cell line by means of homologous recombination;
利用同源重组建立CHO高表达细胞株
3.
Construction of recombinant adeno-viral plasmid bearing hSDF-1α cDNA by homologous recombination in bac-teria and preparation of recombinant adenovirus expressing hSDF-1α;
细菌内同源重组快速构建和制备表达hSDF-1α的重组腺病毒
补充资料:同源重组技术
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性质:见基因打靶
CAS号:
性质:见基因打靶
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