Objective As generally known that non-cytotoxic pro-drug can be transformed into cytotoxic drug by suicide gene,this work is to investigate the effect of Coli cytosine deaminase/5-fluorocytosine suicide gene(CD/5-FC) used alone or combined with irradiation in esophageal carcinoma cell line(EC).

Study of neural stem cells modified by cytosine deaminase gene and its expression in vitro;

Prokaryotic expression of cytosine deaminase and the preparation of polyclonal antibody;

Study on killing efficacy of yeast cytosine deaminase /5 - fluorocytosine gene therapy system on K562B cell line;