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1)  metal-chelating affinity chromatograph(MCAC)
金属鳌合层析
2)  Metal chelate chromatography
金属螯合层析
3)  Metal immobilization affinity chromatography
镍金属螯合层析
4)  Ni-chelating-Sepharose
金属螯合层析
1.
After denaturation and renaturation,the fusion proteins were purified using three chromatographic columns—namely Ni-chelating-Sepharose before and after enzyme digestion,and Sephadex G-50—were operated in series.
2 mmol/L氧化型谷胱甘肽脉冲加样稀释复性;金属螯合层析收集0。
5)  Immobilized metal ion affinity chromatography
金属螯合亲和层析
1.
Purification of recombinant hepcidin fusion protein by immobilized metal ion affinity chromatography;
重组Hepcidin融合蛋白的金属螯合亲和层析纯化
2.
Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography
金属螯合亲和层析法纯化抗乙肝核心抗原单克隆抗体
3.
Immobilized metal ion affinity chromatography(IMAC) is one of the most efficient techniques in biological macromolecular separation and purification which has the advantages of high specificity, high recovery efficiency, high purity and single-step operation.
金属螯合亲和层析具有螯合介质制备简单,吸附容量大,选择性及通用性较好,易于再生,成本低等优点,是分离纯化蛋白质等生物工程产品最有效的技术之一。
6)  metal chelate affinity chromatography
金属螯合亲和层析
1.
Superoxide dismutase(SOD) was preliminarily separated from maize by precipitation with ammonium sulfate and gelfiltration and then purified by metal chelate affinity chromatography(MCAC).
利用硫酸铵分级沉淀、凝胶过滤初步分离了玉米SOD,利用金属螯合亲和层析纯化了SOD。
2.
Isolation and purification of two SOD components(A and B) from maize by metal chelate affinity chromatography and DEAE-cellulose ion-exchange chromatography and determination of their qualities had been carried out in this experiment.
利用金属螯合亲和层析和DEAE-纤维素离子交换层析相结合的方法分离纯化玉米SOD,得到2种达到电泳纯的SOD组分(A、B),并对它们的基本性质进行了初步分析。
补充资料:金属
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性质:具有特有的金属光泽以及良好导电导热性的固态或液态单质。在固态时还具有延展性。大多数元素是金属元素,其特性由元素原子结构和晶体内部结构决定:原子电负性小,吸引电子能力不大,聚集状态时电子流动性大;价层s、p电子少,常形成密堆集晶体结构,如立方面心密堆集、六方密堆集及体心立方堆砌结构。

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