1) protein trans-splicing system
蛋白质反式剪接系统
1.
Therefore a new protein trans-splicing system using an artificial PRP8 split intein from Cryptococcus neoformans serotype AD was successfully constructed and was shown to be efficient in ligation of flanking proteins.
利用此Cryptococcus neoformans AD血清型PRP8断裂蛋白质内含子,成功构建了蛋白质反式剪接系统。
2) protein trans-splicing
蛋白质反式剪接
1.
In this study we explored the intein-mediated protein trans-splicing to deliver a Phe309→Ser mutant full-length fVⅢ (F309SfVⅢ) gene by using a dual-vector system.
本文旨在利用内含肽(intein)的蛋白质反式剪接,研究双载体真核细胞转具有促分泌作用的F309S突变体全长fVⅢ(F309SfVⅢ)基因。
2.
[Objective] By exploring Ssp DnaE intein-catalyzed protein trans-splicing we investigated the ligation of expression product of ATP-binding cassette transporter A1(ABCA1)gene in E.
【目的】利用SspDnaEintein的蛋白质反式剪接技术研究在大肠杆菌中对ABCA1基因表达产物的连接作用。
3.
We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing.
研究利用intein的蛋白质反式剪接功能在大肠杆菌中对凝血VIII因子(FVIII)重链和轻链的连接作用,将B结构域大部分缺失型FVIII(BDD-FVIII)于满足剪接所需的保守性氨基酸Ser1657前断裂为重链和轻链,分别与split mini Ssp DnaB intein的106个氨基酸的N端(Int-N)和48个氨基酸的C端(Int-C)融合,构建到原核表达载体pBV220。
3) protein splicing
蛋白质剪接
1.
everal cases of protein splicing have beenfound recently which is different from so calledRNA splicing.
最近在几种生物体中连续发现了蛋白质剪接现象，由于它与通常所说的ＲＮＡ剪接的区别，人们称这些在蛋白质水平被剪切掉的部分为“蛋白质内含子”。
2.
This process is termed as protein splicing.
这种翻译后的加工过程称为蛋白质剪接(protein splicing)。
3.
Autocatalytic protein splicing mediated by intein is carried out with 100% efficiency in primitive species.
这种含有可选择蛋白质外显子的筛选系统,将蛋白质剪接与卡那霉素抗性相结合,直接从平板上观测剪接结果,成为快速、稳定筛选在异源物种中具有剪接活性蛋白内含子的新手段。
4) protein splicing
蛋白剪接
1.
We want to test whether protein splicing can occur in higher eucaryotic cell,which would play an important role in transgene containment in transgenic plants.
将 4种重组质粒中的aroA In(蛋白内含子Intein插入aroA)融合基因插入 pET 32得到表达载体 pETLEBC、pETLEBT、pETLERC和 pETLERT ,IPTG诱导后 ,SDS PAGE分析显示其能在DE3 中有效表达并发生相应的蛋白剪接。
5) trans-splicing
反式剪接
1.
RNA trans-splicing in many primary eukaryotes has been found,and testified, since it was discovered from splicing of RNA of variable surface glycoprotein (VSG) in trypanosome.
从锥虫可变表面糖蛋白 ( VSG)中发现反式剪接以来 ,已经发现很多低等真核生物体内都存在这种剪接形式。
2.
Protein trans-splicing is a unique posttranslational modification process.
蛋白反式剪接是蛋白翻译后修饰的一种特殊机制,这一反应由断裂型内含肽自我催化完成,不需要酶和其他因子参与。
3.
To construct a trans-splicing ribozyme used to repair mutant green fluorescence protein(GFP)gene,the mutant GFP gene express recombinant plasmids XYQ5/10-pGEM,XYQ5/10-pEGFP-C2 and the trans-splicing ribozyme recombinant plasmid trans-rib-CMV2 were constructed.
为构建修复突变绿色荧光蛋白(GFP)基因的反式剪接核酶,分别构建包含突变的GFP基因的XYQ5/10-pGEM重组质粒、XYQ5/10-pEGFP-C2重组质粒及用于修复该突变基因的反式剪接核酶载体trans-rib-CMV2。
6) protein expression systems
蛋白质表达系统
1.
Recently,some new isotope protein labeling strategies and new protein expression systems have emerged as tools for studying large proteins by sol.
至今已发展的同位素标记技术和蛋白质表达系统已获得了广泛的应用,对蛋白质的核磁共振研究起到了巨大的推动作用。
补充资料：蛋白
蛋白
为食物蛋白和蛋白胨的中间产物，可溶于水，遇热不凝固，可供培养微生物之用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条