1) SL(spliced leader) trans-splicing
SL反式剪接
1.
In the process of SL(spliced leader) trans-splicing,with the help of spliceosome,SL as a mini-exon is added to the 5 -end of pre-mRNA to mature mRNA through the sites SD in SL RNA and SA in pre-mRNA.
在SL反式剪接过程中,生物利用SL RNA分子上的SD位点和pre-mRNA分子上的SA位点,在剪接体的作用下,将SL作为小型外显子加到pre-mRNA上形成成熟的mRNA。
2) trans-splicing
反式剪接
1.
RNA trans-splicing in many primary eukaryotes has been found,and testified, since it was discovered from splicing of RNA of variable surface glycoprotein (VSG) in trypanosome.
从锥虫可变表面糖蛋白 ( VSG)中发现反式剪接以来 ,已经发现很多低等真核生物体内都存在这种剪接形式。
2.
Protein trans-splicing is a unique posttranslational modification process.
蛋白反式剪接是蛋白翻译后修饰的一种特殊机制,这一反应由断裂型内含肽自我催化完成,不需要酶和其他因子参与。
3.
To construct a trans-splicing ribozyme used to repair mutant green fluorescence protein(GFP)gene,the mutant GFP gene express recombinant plasmids XYQ5/10-pGEM,XYQ5/10-pEGFP-C2 and the trans-splicing ribozyme recombinant plasmid trans-rib-CMV2 were constructed.
为构建修复突变绿色荧光蛋白(GFP)基因的反式剪接核酶,分别构建包含突变的GFP基因的XYQ5/10-pGEM重组质粒、XYQ5/10-pEGFP-C2重组质粒及用于修复该突变基因的反式剪接核酶载体trans-rib-CMV2。
3) protein trans-splicing
蛋白质反式剪接
1.
In this study we explored the intein-mediated protein trans-splicing to deliver a Phe309→Ser mutant full-length fVⅢ (F309SfVⅢ) gene by using a dual-vector system.
本文旨在利用内含肽(intein)的蛋白质反式剪接,研究双载体真核细胞转具有促分泌作用的F309S突变体全长fVⅢ(F309SfVⅢ)基因。
2.
[Objective] By exploring Ssp DnaE intein-catalyzed protein trans-splicing we investigated the ligation of expression product of ATP-binding cassette transporter A1(ABCA1)gene in E.
【目的】利用SspDnaEintein的蛋白质反式剪接技术研究在大肠杆菌中对ABCA1基因表达产物的连接作用。
3.
We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing.
研究利用intein的蛋白质反式剪接功能在大肠杆菌中对凝血VIII因子(FVIII)重链和轻链的连接作用,将B结构域大部分缺失型FVIII(BDD-FVIII)于满足剪接所需的保守性氨基酸Ser1657前断裂为重链和轻链,分别与split mini Ssp DnaB intein的106个氨基酸的N端(Int-N)和48个氨基酸的C端(Int-C)融合,构建到原核表达载体pBV220。
4) protein trans-splicing system
蛋白质反式剪接系统
1.
Therefore a new protein trans-splicing system using an artificial PRP8 split intein from Cryptococcus neoformans serotype AD was successfully constructed and was shown to be efficient in ligation of flanking proteins.
利用此Cryptococcus neoformans AD血清型PRP8断裂蛋白质内含子,成功构建了蛋白质反式剪接系统。
6) splicing isomer
剪接异构体反转录多聚酶链式反应
补充资料：反式,反式-1,3-丁二烯-1,4-二羧酸
分子式：C6H6O4
分子量：142.11
CAS号：3588-17-8
性质：熔点290°C (dec.)。沸点320°C。水溶性insoluble。
分子量：142.11
CAS号：3588-17-8
性质：熔点290°C (dec.)。沸点320°C。水溶性insoluble。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条