Hepatitis C virus (HCV) NS3/4A serine protease and NS5B RNA-dependent RNA polymerase are vital for the processing and maturation of viral protein precursors and RNA replication.

Objective To investigate the effects of HCV 3′UTR containing different lengths of PolyU/UC tract on IRES-mediated translation and RNA synthesis by NS5B polymerase.

Yeast expression plasmid containing hepatitis C virus nonstructural gene 5b from infection clone was constructed and transformed into Pichia pastori,constitutive expression of soluble HCV RNA-dependent RNA polymerase(RdRp)was obtained and indicated to be special 64.

The 3D gene encoding RNA-dependent RNA polymerase(RdRp) of foot-and-mouth disease virus was amplified by RT-PCR using pfu ultra~(TM) High-Fidelity DNA polymerase,and the amplicon was cloned into the eukaryotic expression vector pcDNA3.

The full-length NS5 (NS5F) and the NS5 fragment encoding the RdRp, RNA-dependent RNA polymerase, domain (NS5pol) were PCR amplified from a full-length WNV cDNA clone and cloned into the prokaryotic expression vectors pET-28a, respectively.

In the structural proteins, the P1 was the putative RNA-dependent RNA polymerase, which might play an important role in the synthesis of RGDV genomic dsRNAs;The P5 bound with GTP and UTP was a NTP binding protein with RNA guanylyltransferase activity that synthesized a cap structure (mGpppAmpGp) for.

The RNA-dependent RNA polymerase(RdRp) is the viral replicase.

Cloning of RNA-dependent RNA polymerase(RdRp) gene of HCV and its expression in E.coli system;