1) GeneRacer
快速扩增技术
1.
Its full-length was amplified by GeneRacer rapid amplification of cDNA ends.
方法根据本实验室已经克隆的家蝇抗菌肽基因diptericin片段设计1对引物,运用cDNA末端快速扩增技术(GeneRacer)、T-A克隆和序列测定,将所得序列与GenBank中已知的diptericin基因进行同源性分析。
2) RACE
[英][reɪs] [美][res]
cDNA末端快速扩增技术
1.
To understand the mechanism of chick focal adhesion kinase gene(FAK)expression and regulation,the transcription start sites and four kinds alternatively spliced 5′end(5′untranslated region,5′UTR)of FAK mRNA in chick embryo fibroblast cells were identified by RACE.
为了研究鸡局部黏着斑激酶(FAK)基因表达调控的分子机制,运用cDNA末端快速扩增技术确定了鸡胚成纤维细胞FAK基因的转录起始位点,并发现FAK基因mRNA的5′非翻译区(5′UTR)存在4种剪切形式。
2.
Rapid amplification of cDNA ends (RACE) is a polymerase chain reaction (PCR)-based technique which was developed to facilitate the cloning of full-length cDNA 5 and 3 ends after a partial cDNA sequence has been obrained by other methods.
cDNA末端快速扩增技术是一种基于多聚酶链式反应的技术 ,它的发展大大便利了应用其它方法获得的部分cDNA序列后克隆全长cDNA 5’和 3’末端的工作。
3.
This study has cloned Grass carp The full-length cDNA of CRP by the methods of RACE and analysed the sequences, And the CRP tissue distribution was detected in the Grass carp different tissue With the method of RT-PCR,as well as Prokaryotic Expression vector of CRP was suceessfully to be constructed.
本研究采用cDNA末端快速扩增技术(RACE)’对草鱼C反应蛋白基因的cDNA全长序列进行克隆,序列分析,并通过RT-PCR方法研究了草鱼CRP组织分布,同时成功构建了草鱼CRP原核表达重组质粒。
4) rapid technology
快速技术
6) RACE
[英][reɪs] [美][res]
快速cDNA末端扩增
1.
The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).
利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
补充资料：快速
速度快的；迅速：～照相机ㄧ～炼钢ㄧ～行军 ㄧ～育肥。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条