A kind of new-found mutation of hepatitis B virus,clustering mutations in nt 1726-1730 of the core promoter and its clinical significance in patients with chronic hepatitis B;

Significance of core promoter mutation of hepatitis B virus;

Objective:To investigate the relationship between hepatitis B virus(HBV)basic core promoter(BCP)mutation and serum HBV DNA contents as well as cytokines with hepatocellular carcinoma(HCC).

Objective: To study the interaction site of PLAGL2 modulating surfactantprotein-C gene expression Methods: Site-directed mutagenesis was performed to get the SP-C gene promoter mutant(SP-CP-mComclu) and PLAGL2 mutants(PLAGL2-mF6,PLAGL2-mF7);EMSA was performed to identified the ability and specifity of interaction between PLAGL2 or it′s mutant and SP-C gene promoter DNA fragment or it s mutant.