1) PCR-restriction fragment length polymorphism
聚合酶链反应-限制片断长度多态性
2) PCR-RFLP
聚合酶链反应-限制性片断长度多态性
3) Polymerase chain reaction and restriction fragment length polymorphism
聚合酶链反应-限制性片断长度多态性技术
4) polymerase chain reation restriction fragment length polymorphism
聚合酶链式反应-限制性片断长度多态性
5) PCR-RFLP
聚合酶链反应-限制性片段长度多态性
1.
Methods: After gene diagnosis of CSMA for the proband, amniotic fluid of seven pregnancies was collected for fetal DNA extraction and analysis of the survivial motor neuron (SMNt) gene was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method.
方法:通过应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,首先对7例家系中的CSMA先证者进行基因诊断,并检测到其运动神经元存活基因(SMNt)第7、8外显子全部缺失,然后抽取孕妇羊水,盐析法提取羊水中的胎儿DNA,运用PCR-RFLP方法对胎儿SMNt基因第7、8外显子进行检测。
2.
Methods MTHFR gene C677T porlymorphism was detected in 141 subjects,in- cluding 69 in control (CON) group and 72 in cerebral infarction (CI) group by PCR-RFLP technique.
方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测72例脑梗死患者和69例对照者的MTHFR基因C677T多态性分布,并进行对比分析。
3.
Methods:β-Fibrinogen gene G/A-455 polymorphisms have been detected using PCR-RFLP technique in 72 patients with cerebral infarction(CI) and in 69 healthy controls(CON).
方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测脑梗死组(CI组)72例和对照组69例-βFg-455G/A基因的多态性,并测定其血浆纤维蛋白原(Fg)的含量。
6) polymerase chain reaction-restriction fragment length polymorphism
聚合酶链反应-限制性片段长度多态性
1.
Methods PCR primers were designed by create restriction site(CRS) method, then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted to identify four SNPs in MGMT gene.
方法应用碱基错配创造酶切位点原理设计引物,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术判断MGMT基因四个SNP位点多态性。
2.
Objective Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay was applied to molecular typing of resistance-associated esterase genes of Culex pipiens complex.
目的应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测法,建立尖音库蚊抗性酯酶等位基因分型方法。
3.
Methods apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
方法采用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析103例型高脂血症患者和146名血脂正常者的apoE基因型、HDL各亚类组成及相对含量。
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条