1) PCR-RFLP
多聚酶链反应-限制性片段长度多态性
1.
Method: Ninety-six unrelated Uigur Chinese population and 104 unrelated Han population subjects were genotyped for CYP2C19 by PCR-RFLP.
方法:选取96例维吾尔族和104例汉族健康志愿者,抽取静脉血5ml,采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)法进行CYP2C19基因多态性分析,并对维吾尔族与汉族人基因表型和基因频率进行比较。
2.
Polymerse chain reaction-restriction fragment length polymorphisms(PCR-RFLP)techniques were used to analyze TGF-β_1 gene-50.
方法选择110例汉族煤工尘肺患者和110例煤尘接触者为研究对象,采集外周静脉血,应用TGF-β1酶联免疫吸附试验(ELISA)试剂盒检测血清TGF-β1浓度,应用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TGF-β1基因-509位点基因型。
2) PCR-RFLP
聚合酶链反应-限制性片段长度多态性
1.
Methods: After gene diagnosis of CSMA for the proband, amniotic fluid of seven pregnancies was collected for fetal DNA extraction and analysis of the survivial motor neuron (SMNt) gene was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method.
方法:通过应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,首先对7例家系中的CSMA先证者进行基因诊断,并检测到其运动神经元存活基因(SMNt)第7、8外显子全部缺失,然后抽取孕妇羊水,盐析法提取羊水中的胎儿DNA,运用PCR-RFLP方法对胎儿SMNt基因第7、8外显子进行检测。
2.
Methods MTHFR gene C677T porlymorphism was detected in 141 subjects,in- cluding 69 in control (CON) group and 72 in cerebral infarction (CI) group by PCR-RFLP technique.
方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测72例脑梗死患者和69例对照者的MTHFR基因C677T多态性分布,并进行对比分析。
3.
Methods:β-Fibrinogen gene G/A-455 polymorphisms have been detected using PCR-RFLP technique in 72 patients with cerebral infarction(CI) and in 69 healthy controls(CON).
方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测脑梗死组(CI组)72例和对照组69例-βFg-455G/A基因的多态性,并测定其血浆纤维蛋白原(Fg)的含量。
3) polymerase chain reaction-restriction fragment length polymorphism
聚合酶链反应-限制性片段长度多态性
1.
Methods PCR primers were designed by create restriction site(CRS) method, then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted to identify four SNPs in MGMT gene.
方法应用碱基错配创造酶切位点原理设计引物,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术判断MGMT基因四个SNP位点多态性。
2.
Objective Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay was applied to molecular typing of resistance-associated esterase genes of Culex pipiens complex.
目的应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测法,建立尖音库蚊抗性酯酶等位基因分型方法。
3.
Methods apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
方法采用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析103例型高脂血症患者和146名血脂正常者的apoE基因型、HDL各亚类组成及相对含量。
4) polymerase chain reaction restriction fragment length polymorphism
聚合酶链反应-限制性片段长度多态性
1.
Methods APOE genotype was assayed by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP).
方法应用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析112例高脂血症患者和73名正常对照者APOE基因型、HDL各亚类组成及相对含量。
6) Multi PCR-RFLP
多重聚合酶链反应-限制性片段长度多态性
补充资料:开觉自性般若波罗蜜多经
【开觉自性般若波罗蜜多经】
(经名)佛说开觉自性般若波罗蜜多经。四卷,宋惟净等译。
(经名)佛说开觉自性般若波罗蜜多经。四卷,宋惟净等译。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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