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1)  Recombinant endochitinase
重组内切几丁质酶
2)  endochitinase
内切几丁质酶
1.
Studies on the Cloning, Characterization and Expression of Endochitinase Gene from Trichoderma Viride in Escherichia Coli;
绿色木霉内切几丁质酶基因的克隆及其在大肠杆菌中表达的研究
2.
In order to find out the roles of endochitinase during biocontrol process in Chaetomium globosum,a 42 kDa endochitinase gene(chbi) cloned from the genomic DNA of Trichoderma viride was transferred to C.
含有来自绿色木霉Trichoderma viride的内切几丁质酶基因chbi的真菌表达载体pECHBI,通过原生质体转化将该基因导入球毛壳菌Chaetomium globosumCG12菌株,内切几丁质酶活性的测定结果表明,1/5的CG12转化子的内切几丁质酶活性得到了明显提高,PDA平板测定证实毛壳菌转化子对9种病原真菌具有拮抗能力,其中4株转化子的拮抗能力被显著提高。
3.
An endochitinase encoding gene was cloned from the genome DNA of Trichoderma viride.
利用PCR方法从Trichoderma viride的基因组DNA中克隆了一个42kDa的内切几丁质酶基因,扩增的长度为1672bp,其中包含了启动子和mRNA的编码区。
3)  Recombinant chitinase
重组几丁质酶
1.
The fast purification of recombinant chitinase was conducted with Hollow fiber UF membrane and Sephadex G-100, and SDS-PAGE analysis indicated that there was a single band with the right molecular weight.
用7L发酵罐对毕赤酵母(Pichia pastoris)工程菌株NB01进行了重组几丁质酶的诱导表达,通过提高通气量和调整搅拌转速,使溶氧维持在30%以上,NB01可在发酵84h内达到产酶高峰,酶活最高可达48。
4)  endo-chitinase
内切几丁酶
1.
Activity of endo-chitinase in rice plants treated by J-A kept significantly higher compared with untreated control until 21st day, moreover the endo-β-1,3- glucanase activity reached the highest level at 3rd day after J-A spraying on plants, then the activity declined gradually.
测定了用井冈霉素A处理后3、7、14和21天后盆栽水稻植株的海藻糖酶、内切几丁酶和内切β-1,3-葡聚糖酶的活性。
5)  endochitinase
内切壳多糖酶,内切几丁质酶
6)  exochitinase
几丁质外切酶
补充资料:几丁质合成酶抑制剂
分子式:
CAS号:

性质: 几丁质是存在于甲壳纲动物、真菌和昆虫内的一种高聚物。几丁质合成酶是几丁质生物合成中的关键酶,而由微生物产生的几丁质合成酶抑制剂能抑制该酶的活性,阻止几丁质的生物合成,从而抑制真菌生长或阻止昆虫幼虫和蛹蜕皮达到杀虫效果。由于哺乳类动物没有几丁质代谢系统,所以筛选几丁质合成酶抑制剂,有望开发出新型的对人低毒无害的抗真菌剂和杀虫剂。

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