1) Real-time relative quantitative PCR
实时相对定量PCR
2) relative quantitative real time RT-PCR
荧光实时相对定量RT-PCR
1.
In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.
为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
3) realtime PCR/RT-PCR
实时定量PCR/RT-PCR
4) real-time quantitative PCR
实时定量PCR
1.
Real-time quantitative PCR in determination of telomere length;
荧光实时定量PCR检测端粒长度
2.
Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;
IgH重排的实时定量PCR检测及反应参数研究
3.
Principles of real-time quantitative PCR and its application in plant pathology;
实时定量PCR的原理及其在植物病理学研究中的应用
5) Quantitative real-time PCR
实时定量PCR
1.
287 Gy·min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized,the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.
min-1,总剂量7Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。
2.
The design principles and application in medicine ,molecular biology, food testing of the quantitative real-time PCR were reviewed in this paper.
笔者对实时定量PCR试验设计原则及其在医学、分子生物学、食品检测的应用上进行了概述。
6) RT-PCR
实时定量PCR
1.
Establishment of RT-PCR detecting relative expression of sBD-1-mRNA in Mongolian sheep
蒙古绵羊β-防御素基因相对表达水平实时定量PCR方法的建立
2.
Method:IGF-IR mRNA expression in marrow cells from patients with MDS and normal controls was quantitatively determined by real time reverse transcript polymerase chain reaction(real time RT-PCR).
方法:用实时定量PCR(real time RT-PCR)方法检测胰岛素样生长因子-I受体mRNA的表达量。
补充资料：高压荧光灯用荧光粉
分子式：
CAS号：
性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO4:Eu，Y(V，P)O4:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO4白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。
CAS号：
性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO4:Eu，Y(V，P)O4:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO4白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条