Nowdays,mostly studies about molecular biology of PRRSV is its major structural protein, especially the GP5 of PRRSV, In our laboratory,we had constructed the MuLV-GP5 by pseudotype system,but the study on the secondary structural protein is more less.

Virus protein VP2 is a minor structural protein of rabbit hemorrhagic disease virus(RHDV).

Sequence polymorphism in the main structural protein genes of two strains of Severe Acute Respiratory Syndrome(SARS)-associated coronaviruses;

In this study,the total RNA of cells-PRRSV was extracted,according to VR2332 and CH-la strain,designed six pairers GP2-1/GP2-2,GP3-1/GP3-2,GP4-1/GP4-2,GP5-1/GP5-2,M1/M,N1/N2, structural protein genes fragments of porcine reproductive and respiratory syndrome virus(PRRSV) XJPRRSV1/03 strain were amplified by RT-PCR,cloned into pMD-18T vector and pGM-T vector and sequenced.

Molecular cloning and prokaryotic expression of structural protein VP3 gene of IBDV;

Eukaryotic expression of porcine reproductive and respiratory syndrome virus structural protein genes ORF3,ORF5 and ORF6;

Cloning of the structural protein gene and study of cell-recognition site of foot-and-mouth disease virus(Akesu/O/58);

Objective:To construction the cloning and yeast expression vectors containing ORF5、ORF6、ORF7 structural protein gene of equine arteritis virus and lay foundation for expressing these recombinant protein.

In recent years, the research work on TGEV was focused on its structural proteins and there had no report on the non-structural protein gene and non-encoding regions in China.