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1)  antisense plant expression vector
反义植物表达载体
1.
【Objective】The research aimed to obtain HKD2 domain in PLD gene and construct its antisense plant expression vector,so as to study the effect of PLDα gene on the storage property,【Method】total RNA was extracted from the mature fruit of strawberry,then cDNA was amplified by reverse transcriptase polymerase chain reaction(RTPCR) and to clone T-A into pMD19-T simple vector.
【目的】获得草莓磷脂酶Dα(PLDα)的HKD2功能区基因序列构建反义植物表达载体
2)  plant antisense expression vector
植物反义表达载体
3)  plant expression vector
植物表达载体
1.
Construction of plant expression vector of human interleukin-12;
人白介素-12植物表达载体的构建
2.
Cloning of prME in Japanese encephalitis virus and construction of its plant expression vector;
日本脑炎病毒(JEV)prME的克隆及其植物表达载体构建
3.
Construction of the plant expression vector for the truncated fragments of SAG1 gene of Toxoplasma gondii;
弓形虫SAG1基因截短片段植物表达载体的构建
4)  plant expression vectors
植物表达载体
1.
Construction of plant expression vectors containing two anti-fungal and one anti-herbicide genes and their expression in transgenic tobacco
抗真菌和抗除草剂基因多价植物表达载体构建及对烟草遗传转化的研究
2.
This paper uses gene engineering technique to construct plant expression vectors of stilbene synthase gene appended by fruit special expression promoter,and introduces these vectors into agrobacterium strains LBA4404 and EHA105,and uses PCR and restriction enzyme digestion technique to identify these strains.
本研究利用基因工程技术构建了附加果实特异表达启动子的芪合酶基因植物表达载体,并通过农杆菌直接转化技术将其转入LBA4404、EHA105,并采用PCR及限制性酶切分析对所获得的农杆菌工程菌株进行鉴定。
5)  plant express vector
植物表达载体
1.
Construction of multivalent plant express vector of chi,rip and DREB1A gene;
rip、chi及DREB1A多价基因植物表达载体的构建
2.
Further more,OsCDPK7 gene was cloned into plant express vector cassette pBE12 and pB29A.
进一步将OsCDPK7基因分别克隆至植物表达载体卡盒pBE12和pB29A上,构建了分别由E12启动子、rd29A启动子调控的OsCDPK7基因植物表达载体pBC7E12和pBC729A。
3.
Further more OsMAPK4 gene was cloned into plant express vector cassette pBCE12 and pBC29A.
进一步将OsMAPK4基因分别克隆至植物表达载体卡盒pBCE12和pBC29A上,构建了分别由E12启动子、rd29A启动子调控的OsMAPK4基因植物表达载体pBME12和pBM29A。
6)  GO plant expression vector
GO植物表达载体
补充资料:植物克隆载体
分子式:
CAS号:

性质:植物基因操作中之携带外来DNA的载体,并以可遗传的方式将它嵌入植物细胞之内。天然的载体有根癌根杆菌(Agrobacterium tumefaciens)的Ti质粒或发根农杆菌(A. rhizogenes)的Ri质粒以及DNA植物病毒。现在因Ti或Ri质粒太大,不易操作,常取其DNA复制及引发癌肿的基因与普通细菌质粒组成穿梭质粒,便于先在细菌(尤其是大肠杆菌)中操作、扩增,然后再引入植物。

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