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1)  the full-length infectious cDNA clone
全长感染性cDNA克隆
2)  infectious full-length cDNA clone
感染性全长cDNA克隆
1.
Construction and identification of infectious full-length cDNA clone of dengue type 2 virus isolated in China;
登革2型病毒中国分离株感染性全长cDNA克隆的构建与鉴定
2.
Methods On the basis of infectious full-length cDNA clone pD212 of DEN2-43 strain virus, the deletion mutants were constructed by fusion PCR, from which the rescued viruses with deletion mutation of capsid protein were generated by transcription in vitro and electroporation.
方法在DEN2-43株病毒感染性全长cDNA克隆的基础之上,利用融合PCR技术构建衣壳蛋白基因缺失的全长cDNA,将其线性化并体外转录成RNA后,经电穿孔法导入宿主细胞获得衣壳蛋白缺失突变病毒。
3)  full length infectious cDNA
侵染性全长cDNA克隆
4)  infectious cDNA clone
感染性cDNA克隆
5)  full-length cDNA clone
全长cDNA克隆
1.
The full-length cDNA clone pSCMV of SCMV-BJ was constructed from three overlapping fragments cDNA clones downstream from the bacteriophage T7 promoter.
以接种甘蔗花叶病毒北京分离物(SCMV-BJ)的发病玉米叶片为材料,RT-PCR扩增得到三段部分重叠并覆盖全长的片段,采用拼接的方法获得了T7启动子控制下的SCMV-BJ全长cDNA克隆pSCMV,并将该克隆的体外转录物摩擦接种至健康的玉米植株上。
2.
The present article describes the establishment of an infectious full-length cDNA clone of PRRSV BJ-4 by utilizing reverse genetic manipulation technology.
本研究利用反向遗传学操作技术,构建了PRRSV BJ-4株的全长cDNA克隆,并对全长cDNA克隆中存在的23个突变位点进行了回复突变,继而对突变后的全长cDNA克隆体外转录体的感染性进行了研究。
3.
[Methods] Three fidelity DNA fragments covering the full genome of DHV type1 CL strain were amplified by RT-PCR,and inserted into pBR322 vector,resulting in the full-length cDNA clone BR-CL.
【方法】用RT-PCR方法扩增出覆盖整个1型鸭肝炎病毒CL株基因组3个忠实性片段,并按顺序组装进载体pBR322中,获得全长cDNA克隆(BR-CL)。
6)  full-length cDNA
全长cDNA克隆
1.
Construction of a full-length cDNA clone of PTV Swine/CH/IMH/03;
猪捷申病毒Swine/CH/IMH/03全长cDNA克隆的构建
补充资料:放射性标记单克隆抗体
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性质: 用放射性核素标记的抗肿瘤及其相关抗原的单克隆抗体,用于放射免疫显像(用发射γ放射性核素标记的)和放射免疫治疗(用发射α,β放射性核素标记的)。放射性标记单克隆抗体有直接法和用双功能联结剂的间接法。现已用于肝癌、肺病、结肠癌等的诊断的治疗中。

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