1) infectious cDNA clone
感染性cDNA分子克隆
1.
In order to research high variability and host tropism,we sequenced the full length genome of FMDV O/CHINA/99 strain isolated from cattle, and generated an infectious cDNA clone of the strain.
为了研究其致病机制、抗原变异的分子机理和宿主嗜性,我们首次构建了FMDV牛源泛亚型O/CHINA/99株的3株不同感染性cDNA分子克隆。
3) Infectious molecular clone
感染性分子克隆
1.
Immunological characteristics of equine infectious anemia virus derived from infectious molecular clone;
感染性分子克隆衍生的马传染性贫血病毒的免疫学特性
2.
In the study,the LTRs of an infectious molecular clone derived from EIAV donkey leukocyte-attenuated vaccine virus (DLA) were substituted with those of EIAV L strain,a virulent EIAV from which D.
为了探讨LTR在EIAV病毒复制和转录过程中的作用,并进一步研究EIAV的致病和免疫机制,用EIAV强毒L株LTR置换了以前构建的EIAVDLA(弱毒)感染性分子克隆中的LTR,构建了马传贫强/弱毒嵌合分子克隆,并获得了具有感染性的强/弱毒嵌合病毒。
3.
In this study,an NspV site was introduced into the S2 gene of pOK8266, an infectious molecular clone derived from EIAV vaccine virus,and then a 6_histidine tag was introduced into NspV site,generating pOK8266_HIS.
本研究以已构建的马传染性贫血驴白细胞弱毒疫苗株的感染性分子克隆(pOK8266)为基础,在其S2基因内引入NspV酶切位点,将人工合成的编码6个组氨酸的寡核苷酸插入NspV位点,获得带有组氨酸标签的重组质粒pOK8266_HIS。
4) infectious full-length cDNA clone
感染性全长cDNA克隆
1.
Construction and identification of infectious full-length cDNA clone of dengue type 2 virus isolated in China;
登革2型病毒中国分离株感染性全长cDNA克隆的构建与鉴定
2.
Methods On the basis of infectious full-length cDNA clone pD212 of DEN2-43 strain virus, the deletion mutants were constructed by fusion PCR, from which the rescued viruses with deletion mutation of capsid protein were generated by transcription in vitro and electroporation.
方法在DEN2-43株病毒感染性全长cDNA克隆的基础之上,利用融合PCR技术构建衣壳蛋白基因缺失的全长cDNA,将其线性化并体外转录成RNA后,经电穿孔法导入宿主细胞获得衣壳蛋白缺失突变病毒。
5) genomic infectious cDNA clone
基因组感染性cDNA克隆
补充资料:分子克隆法
分子式:
分子量:
CAS号:
性质:见基因工程。
分子量:
CAS号:
性质:见基因工程。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条