1) enzyme linked immunosorbent assay
酶链免疫吸附法
1.
Methods The serum HGF levels of 100 patients with breast cancer were measured by enzyme linked immunosorbent assay before treatment and the relationship between changes in serum HGF levels and age,expressions of estradiol receptor,tumor size,histologic grade,lymphonodus metastasis and TNM stage were analyzed.
方法应用酶链免疫吸附法检测100例可手术乳腺癌患者治疗前的血清HGF水平,比较年龄、雌激素受体表达情况、肿瘤大小、组织学分级、淋巴结状况及TNM分期与HGF关系。
2) ELISA
[英][i'laizə] [美][ɪ'laɪzə]
酶联免疫吸附法
1.
Development of ELISA System to Determine Ochratoxin A(OTA);
检测赭曲霉毒素A(OTA)的酶联免疫吸附法(ELISA)体系的建立
2.
ELISA and its applications in food analysis;
酶联免疫吸附法及其在食品分析中的应用
3.
Enzyme-linked immunosorbent(ELISA) and micropartical immunoassay(MEIA) for detecting HBsAb;
酶联免疫吸附法和微粒子酶免疫分析法检测HBsAb的比较
3) enzyme-linked immunosorbent assay
酶联免疫吸附法
1.
Determination of isoproturon in water by enzyme-linked immunosorbent assay;
酶联免疫吸附法测定水中异丙隆
2.
Determination of sufentanil in human serum by using enzyme-linked immunosorbent assay;
酶联免疫吸附法测定人血清舒芬太尼浓度
3.
Objective To detect the panel reactive antibodies(PRA)in children with β-thalassemia major by enzyme-linked immunosorbent assay(ELISA)for investigating the level of sensitization and clinical significance of transfusion dependant.
目的采用酶联免疫吸附法(ELISA)检测重型β地中海贫血患儿的群体反应性抗体(panel reactive antibodies,PRA),探讨反复输血的地中海贫血患儿体液致敏水平及其临床意义。
4) Enzyme linked immunosorbent assay
酶联免疫吸附法
1.
The plasma concentrations of r-SAK were detected by dual antibody enzyme linked immunosorbent assay method.
方法24例健康受试者随机分为3组(5,10,15mg剂量组),建立双抗体酶联免疫吸附法(ELISA),测定r-SAK的血药浓度,用3P97药代动力学软件进行数据处理。
2.
Methods Enzyme linked immunosorbent assay method was used to test the serum anti-tuberculosis antibody of each of the 123 new smear positive pulmonary tuberculosis patients whose results of bacteria culture of sputum showed mycobacterium tuberculosis or mycobacterium .
方法采用酶联免疫吸附法检测痰结核菌培养为人型或牛型结核分枝杆菌的初治涂阳肺结核患者共123例的血清抗结核抗体,分析抗结核抗体阳性率并将检验结果与患者性别、年龄、病变范围、空洞情况、病程、合并症、痰菌量、菌型等的关系进行统计学分析。
3.
Methods The levels of serum osteopontin in 120 patients with lung cancer in different TNM stages,23 pulmonary inflammatory pseudotumor(PIP) as disease controls and 49 out-patients physical examination normal subjects as healthy controls were measured by enzyme linked immunosorbent assay(ELISA),and further comparison of serum osteopontin level before and after treatment .
方法选择不同临床TNM分期的肺癌患者120例作为肺癌组,同期肺炎性假瘤患者23例作为疾病对照组,49例门诊体检正常者作为健康对照组,应用酶联免疫吸附法(ELISA)进行血清骨桥蛋白水平检测。
5) enzyme-linked immunosorbent assay(ELISA)
酶联免疫吸附法
1.
Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect serum anti CCP antibody in patients with RA.
方法利用酶联免疫吸附法(ELISA)测定患者抗CCP抗体在RA中的含量。
补充资料：酶联免疫吸附测定法
酶联免疫吸附测定法
诊法。免疫标记技术之 一。用于测定抗精子抗体。原理为将欲测血清与相应的固相抗原形成免疫复合物，由标记上 酶(过氧化氢酶、碱性磷酸酶、尿素酶等)的二抗检测。利用这结合的酶使基质(邻苯二胺、 对硝基苯酚、尿素等)转化为色素原，出现的颜色反应以目测或分光光度计测出。方法：用 PBS洗涤精子，将其密度调至25×106/50μl。取50μl加到微量滴定盘的每一凹孔中， 以025%戍二醛固定。用含005%Tween20(表面活性剂)的PBS(PBS-Tween)洗3次，加入50 μl稀释的血清或精浆(1∶4稀释)，在37℃下孵育60分钟后，用PBS-Tween液将凹孔洗3次， 加入50μl，1∶100稀释的联结有磷性磷酸酶的羊抗人免疫球蛋白，经37℃60分钟孵育，再 用PBS-Tween液洗3次，加入250μl含1mg/ml磷酸对硝基苯酚的005M碳酸钠缓冲液，3 7℃60分钟孵育后，每孔加入50μl3NNaOH以中止反应。用分光光度计在400nm测定吸收光 谱。含磷酸对硝基苯酚和3NNaOH作为空白。本法优点在于不需新鲜精子，适宜大量标本测定 ，但与无关抗原的非特异性反应是主要问题。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条