Optimizing Yanbian Cow Oocytes Mature in vitro and Cleavage System after Nuclear Transfer Based on Uniform Design

To establish an optimal conditions for in vitro maturation of ovine oocytes,we studied the effects of EGF,IGF-I and the union use of both of them on in vitro maturation and cleavage of ovine oocytes.

This paper studies the embryonic development of Spodoptera exigua(Hübner) including the cleavage, the formation of blastoderm and germ band, and the differentiation of germinal layers.

The ultrastructural characteristics of the bovine preantral follicles isolated freshly;

Isolation and culture of preantral follicles from buffalo fetal, heifer and adult cow ovaries;

Progress in the research on preantral follicles of animals;

The Study on the distributing and histomorphology of buffalo preantral follicles;

Study on the isolation of buffalo and mouse preantral follicles;

Effect of nerve growth factor on in vitro development of bovine preantral follicle;