objective To develop the technology of DNA microarray for orthopoxvirus detection, so as to use it for early diagnosis of orthopoxvirus-induced diseases.

Variola virus belongs to the genus of orthopoxvirus, and there are some other infectious members, such as Vaccinia virus, cowpox virus, and monkeypox virus.

Design and preparation of oligonucleotide microarray for vaccinia virus detection;

Construction and Identification of Eukaryotic Expression Vector of Vaccinia Virus Expressing Human Interleukin-2 Gene;

Coexpression of HIV-1p24gag and hIL-6 gene in recombinant vaccinia virus;

Adjuvant effects of chicken IL-1β,IL-2,IFN-γ,MGF expressed by recombinant fowlpox viruses used in combination with recombinant vaccines against Newcastle disease virus;

Protection of chickens against IBV field strain with a recombinant fowlpox virus co-expressing IBV S1 gene and ChIFNγ gene;

Self-excising reporter gene in recombinant Fowlpox virus expressing H5 hemagglutinin gene of influenza A virus using Cre-loxp systerm;