Establishment of real-time PCR assays for detection of monkeypox virus

Objective: The orthopox viruses that are high pathogenic for human include variola virus(VARV, smallpox virus) and monkeypox virus(MPV).

0 software according to the entire F3L gene sequence of monkeypox virus in GenBank to develop a real-time PCR assay for rapid identification of monkeypox virus A typical"S"curve can be got when the artifical F3L gene sequence was used as a target,and the detection limits were 6.

Design and preparation of oligonucleotide microarray for vaccinia virus detection;

Construction and Identification of Eukaryotic Expression Vector of Vaccinia Virus Expressing Human Interleukin-2 Gene;

Coexpression of HIV-1p24gag and hIL-6 gene in recombinant vaccinia virus;

Adjuvant effects of chicken IL-1β,IL-2,IFN-γ,MGF expressed by recombinant fowlpox viruses used in combination with recombinant vaccines against Newcastle disease virus;

Protection of chickens against IBV field strain with a recombinant fowlpox virus co-expressing IBV S1 gene and ChIFNγ gene;

Self-excising reporter gene in recombinant Fowlpox virus expressing H5 hemagglutinin gene of influenza A virus using Cre-loxp systerm;

Detection of mouse immunity induced by HIV transmembrane modified DNA vaccine combined with poxvirus by ELISPOT and its specific CTL function;

Isolation and Identification of a Mouse Poxvirus and Its Infectivity;

An outbreak of poxvirus infection was occurred in twenty bought KM and BALB/c mice .

The rabies virus strain ERA was passaged and reproduced in BHK 21 cell, and it was observed by electron microcopy The result shows that there are too many pox virus except for a few of target viruses, it is mouse poxvirus by ELISA test The mouse poxviruses possible came from focus mouse which had ever contaminated poxvirus, for it used recovery virulence experiment was activate

VZV shape change in 2BS cell infected HAV;

Objective To study the propagation dynamics of hepatitis A(HAV)and varicella-zoster viruses(VZV) in the human embryo lung diploid cell(2BS) after mixed infection with the 2 kinds of viruses.