1) yeast petite mutation
酵母小菌落突变
2) petite mutant
小菌落突变体
3) petite colony mutant
小菌落突变型
4) small colony variants
小菌落突变株
1.
Isolation and identification of a Staphylococcus aureus small colony variants
一株金黄色葡萄球菌小菌落突变株的分离鉴定
2.
Phenotypically, small colony variants have a slow growth rate, atypical colony morphology and unusual biochemical characteristics, making them a challenge for clinical microbiologists to identify.
小菌落突变株是一种具有独特表型及致病特征且生长缓慢的细菌亚群。
5) yeast colony PCR
酵母菌落PCR
1.
Recombinants were identified by yeast colony PCR, then were induced by methanol and detected by SDS-PAGE analysis.
berezovskii)白介素-2(interleukin-2,IL-2)到酵母表达载体pPIC9K,SalⅠ酶切后转化毕赤酵母GS115,G418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,SDS-PAGE分析,结果在诱导至第6d的酵母上清浓缩液中,检测到与预测林麝IL-2分子量相接近的,分子量约为18Ku的诱导表达带;TRIzol提取表达期间的酵母总RNA,并通过RT-PCR扩增IL-2mRNA,发现表达期的重组酵母细胞中存在IL-2mRNA,而对照没有检出,表明林麝IL-2存在低水平的表达。
2.
Recombinants were identified by yeast colony PCR.
5K上,SalI线性化后转化毕赤酵母GS115,418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,Tricine-SDS-PAGE分析,结果在诱导第2d的酵母裂解液中检测到与预期的SMAP-29分子量接近,约3。
6) yeast type colony
酵母型菌落
补充资料:大肠杆菌不同生化突变型菌落(显示不同颜色)
乔守怡提供
[图]

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