1) relative quantity RT-PCR
相对定量RT-PCR
1.
In order to elucidate the mechanism of its promoting growth further, effects of cyadox on the expression of IGF-1 and EGF mRNA in the primary culture piglet hepatocytes and livers were studied in this article by relative quantity RT-PCR methods.
本文运用相对定量RT-PCR方法,研究喹赛多对原代培养猪肝细胞以及仔猪肝脏组织中胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)和表皮生长因子(Epidermal growth factor,EGF)mRNA表达的影响,探讨喹赛多抗菌促生长的机理。
2) relative quantitative real time RT-PCR
荧光实时相对定量RT-PCR
1.
In this research,the small interfering RNAs(siRNAs) obtained by in vitro transcriptional methods were applied to suppress the expression of chTERT mRNA in MDCC-MSB1 cells by relative quantitative real time RT-PCR,and to evaluate the biological consequences of chTERT down-regulation on M.
为通过体外转录的小干扰RNA(Small interference RNA,siRNA)抑制马立克氏病淋巴瘤细胞(MDCC-MSB1)中鸡端粒酶反转录酶(Chicken telomerase reverse transcriptase,chTERT)mRNA的表达,探讨chTERT在MDCC-MSB1中的作用,本试验设计了针对chTERT mRNA的特异siRNA序列(siRNA-1、siRNA-2、siRNA-3),利用T7 RNA聚合酶体外转录系统,制备21 bp的siRNA分子,通过脂质体介导转染MDCC-MSB1细胞,采用荧光实时相对定量RT-PCR方法检测转染细胞chTERT基因mRNA的相对表达量,流式细胞仪测定转染细胞周期变化情况。
3) quantitative RT-PCR
定量RT-PCR
1.
Quantitative RT-PCR in detecting the apoptosis associated gene expression in penumbra area of cerebral infarction in rats;
用定量RT-PCR方法检测脑梗死半影区内凋亡相关基因的表达
2.
Using Immunomagnetic Beads and Quantitative RT-PCR for Detecting Micrometastasis in Peripheral Blood among Patients with Gastric Cancer;
联用免疫磁珠及定量RT-PCR检测胃癌外周血微转移
3.
Objective The purpose of this study is to determine the expression level of CK19 in sentinel lymph nodes(SLN)of breast cancer by quantitative RT-PCR,and to improve the detection rate of micrometastasis in breast cancer.
目的探讨定量RT-PCR法检测细胞角蛋白(CK19)在乳腺癌前哨淋巴结(SLN)中的表达,提高前哨淋巴结活检中微转移的检出率。
5) semi-quantity RT-PCR
半定量RT-PCR
1.
In the present study,a semi-quantity RT-PCR for detection of abundance of liver PEPCK-C mRNA in peripartum dairy cows was established.
建立了奶牛肝PEPCK-C mRNA丰度的半定量RT-PCR检测方法,其优化的PCR反应条件(25μL)为:57℃退火、2。
2.
Material and Methods By RT-PCR(semi-quantity RT-PCR,SQ-PCR), hOCT1 gene mRNA levels were detected from 9 couples of (18) samples.
对象及方法：采用半定量RT-PCR(semi-quantity RT-PCR，SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测，并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果，分析其水平变化和与疾病缓解或伊马替尼耐药相关性。
3.
Expression analysis of CHI of Kyoho grape was carried out by semi-quantity RT-PCR, using grape actin gene as an internal control.
试验从一年生巨峰扦插苗中克隆了CHI基因,并采用半定量RT-PCR技术,以actin基因为内参,分析了CHI基因在巨峰葡萄果实不同发育阶段及组织的表达。
6) semi-quantitative RT-PCR
半定量RT-PCR
1.
Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR;
半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况
2.
Establishment of the semi-quantitative RT-PCR system of nonexpressor of pathogenesis-related genes 1(NPR1) gene in sweet potato(Ipomoea batatas Lam.);
甘薯NPR1基因半定量RT-PCR检测方法的建立
3.
Aplication of Semi-Quantitative RT-PCR in Analysis Gene Expression Level;
半定量RT-PCR法在检测基因表达水平中的应用
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条