1) Human breast cell line MDA-MB-231 and MDA-MB-435
MDA-MB-231 and MDA-MB-435细胞系
2) MDA-MB-231 cells
MDA-MB-231细胞
1.
Apoptosis and DNA damage in MDA-MB-231 cells induced by total synthetic Jaspine B;
全合成Jaspine B诱导人乳腺癌MDA-MB-231细胞凋亡及DNA损伤
2.
Methods: The inhibitory effect of different concentration of baicalin on the MDA-MB-231 cells was measured by MTT method.
方法:MTT法测定不同剂量黄芩苷对MDA-MB-231细胞株增殖的抑制作用;透射电镜检测细胞凋亡及黄芩苷的作用;流式细胞术分析黄芩苷对MDA-MB-231细胞周期变化的影响;RT-PCR技术分析黄芩苷对MDA-MB-231细胞bcl-2、baxmRNA水平表达的影响。
3.
Methods:MDA-MB-231 cells were cultured with As2O3 diflerint cokcenfrations.
方法:将不同浓度的As2O3与MDA-MB-231细胞共培养不同时间后,MTT比色法检测As2O3对MDA-MB-231细胞的生长抑制作用;TRAP-PAGE-银染法检测As2O3作用后细胞端粒酶活性的改变;RT-PCR法测定MDA-MB-231细胞hTERT-mRNA的表达。
3) MDA-MB-231
MDA-MB-231细胞
1.
Effect of 1,25-dihydroxyvitamin D_3 and Tamoxifen on cell cycle distribution and apoptosis of MDA-MB-231 cells transfected with ERα expression vector containing vitamin D response element;
1,25二羟维生素D_3联合他莫西芬对转染VDRE-Tk-ERα表达载体的MDA-MB-231细胞周期和凋亡的影响
2.
Objective To study the pro-apoptotic effects of 1,25 dihydroxyvitamin D3 in combination with Tamoxifen on MDA-MB-231 cells transfected with ERα expression vector containing VDRE elements and associated molecular mechanism.
方法将本室构建的含有4个拷贝维生素D反应元件(VDRE)和胸苷激酶启动子(Tk)的VDRE-Tk-ERα表达载体转染到MDA-MB-231细胞中,利用免疫荧光法检测1,25二羟维生素D3对雌激素受体α(ERα)的诱导表达并通过末端原位法检测1,25二羟维生素D3联合他莫西芬诱导转染该表达质粒的MDA-MB-231凋亡诱导效应。
4) MDA-MB-231 cell
MDA-MB-231细胞
1.
Objective-To establish the nude mouse model of carcinoma of breast MDA-MB-231 cell,and study its biological characteristics.
目的-建立人乳腺癌MDA-MB-231细胞株裸小鼠模型,研究其生物学特性,观察MDA-MB-231乳腺癌细胞在移植前后的形态学变化。
2.
Currently, the conditioned mediums of MDA-MB-231 cells, bone marrow endothelial cells, cultured- sciatic nerves and hepatocytes get more attention.
当前,国内外研究较多的有乳腺癌细胞系MDA-MB-231细胞、骨髓内皮细胞、坐骨神经和肝细胞等的条件培养液。
3.
After identification of restriction endonuclease and sequencing, the recombinant plasmid and empty plasmid were transfected into MDA-MB-231 cells by Lipofectamine TM 2000, respec-tively.
[方法]从pOTB7-OPG上获得OPG基因片段并用PCR方法扩增,将其连接于真核表达质粒pIRES2-EGFP,酶切鉴定及测序后,转染MDA-MB-231细胞,以G418加压筛选,对转染细胞行单克隆化,稳定转染细胞行RT-PCR和Westernblot检测,确定其OPGmRNA和蛋白表达情况,MTT法检测过表达OPG对MDA-MB-231细胞生长速率的影响。
5) MDA-MB-435 cells
MDA-MB-435细胞
1.
High-efficient genetic transfection of CD41~+,UT7,U937 and MDA-MB-435 cells with a recombined murine stem cell retroviral vector;
重组小鼠干细胞逆转录病毒载体高效基因转染CD41~+、UT7、U937和MDA-MB-435细胞
补充资料:MDA
孟烷二胺简称MDA,分子量170.3。透明液体,密度(23℃)0.91,粘度(25℃)19mPa·s。熔点-45℃,沸点107~126℃。
用作环氧树脂的固化剂,参考用量22phr,固化条件80℃/2h或130℃/30min。固化物热变形温度148~158℃。
用作环氧树脂的固化剂,参考用量22phr,固化条件80℃/2h或130℃/30min。固化物热变形温度148~158℃。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条