实时荧光定量 RT-PCR,real-time fluorescent quantitative RT-PCR,在线英语词典,英文翻译,专业英语

1) real-time fluorescent quantitative RT-PCR

实时荧光定量 RT-PCR

2) real-time fluorescent quantitative RT-PCR

实时荧光定量RT-PCR

Expressions of bone metastasis genes in nasopharyngeal carcinoma by real-time fluorescent quantitative RT-PCR;

实时荧光定量RT-PCR检测鼻咽癌骨转移相关基因表达的研究

Establishment of a real-time fluorescent quantitative RT-PCR assay for the detection of chicken IFN-γ gene;

鸡γ-干扰素实时荧光定量RT-PCR检测方法的建立

Methods Using real-time fluorescent quantitative RT-PCR to analyze the expressions of DNMT1,DNMT3A and DNMT3B in normal liver cell line,pericacinoma cell line and hepatocellular carcinoma cell line respectively.

方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。

3) Real-time fluorescence quantitative RT-PCR

实时荧光定量RT-PCR

Methods After treatment of cultured 16HBE cells with NiSO_4 or NiSO_4 adding FJD drug serum,the variation of the free radicals and 8-oxo-dGTPase expression were detected by laser scanning confocal microscopy(LSCM) and real-time fluorescence quantitative RT-PCR,respectively.

方法体外培养的16HBE细胞分别经NiSO4暴露及NiSO4加扶正解毒汤含药血清共同处理后,采用激光扫描共聚焦显微镜(LSCM)及实时荧光定量RT-PCR检测自由基含量及8-oxo-dGTPase表达的变化。

Conclusion Real-time fluorescence quantitative RT-PCR used to detect the expression of .

目的建立实时荧光定量RT-PCR方法检测SurvivinmRNA的表达。

Based on the GenBank sequence data, one TaqMan-MGB probe and the corresponding primers were designed to construct a standard for real-time fluorescence quantitative RT-PCR to detect the expression of hsp70 in B.

为了探讨热激蛋白在B型烟粉虱Bemisiatabaci B-biotype抵抗高温中的作用,以含有B型烟粉虱热激蛋白基因hsp70片段的质粒为模板,用TaqMan-MGB探针和特异性引物构建了B型烟粉虱hsp70实时荧光定量RT-PCR检测体系,并检测了37℃~45℃高温胁迫及气温变化时B型烟粉虱成虫体内hsp70表达的情况。

4) real time RT-PCR

实时荧光定量RT-PCR

One step RT-PCR technique and Real time RT-PCR technique are applied in evaluating the correlation between rate of mRNA degradation in SD rats and postmortem intervals, especially in advanced stage PMI.

目的：本研究从基因库中筛选出两种稳定表达于不同个体与组织细胞中的看家基因GAPDH和β-actin，采用实时荧光定量RT-PCR技术研究大鼠死后不同时间和不同脏器中两种看家基因的mRNA降解规律，建立mRNA降解的程度与死亡时间关系的回归方程，探索用于晚期死亡时间推断较为理想的组织，并试图建立标准化的RNA提取与定量检测方法，希望能最终应用于法医学实践中。

5) Real-time RT-PCR

实时荧光定量RT-PCR

Establishment of Real-time RT-PCR for IFN-γ mRNA in Duck;

鸭IFN-γ mRNA实时荧光定量RT-PCR方法的建立

The CAT1 mRNA abundance was determined by real-time RT-PCR using SYBR Green I RT-PCR mix Kit.

以18S基因为内标,用实时荧光定量RT-PCR法(SYBR GreenⅠ试剂盒)检测CAT1 mRNA在60日龄长白猪十二指肠、空肠、回肠和结肠表达的组织特异性,以及在长白和蓝塘猪不同日龄其十二指肠、空肠、回肠表达的发育性变化。

The detection limit of the real-time RT-PCR assays was 10 RNA copies per reaction mixture.

分别设计HCoV-NL63和HCoV-HKU1特异的引物与荧光标记探针,并合成含靶基因的模板RNA,建立常规RT-PCR方法与实时荧光定量RT-PCR方法,对其灵敏性、特异性和可重复性以及用于临床样本的适用性等进行平行比较评价。

6) real-time fluorescence quantitative RT-PCR

实时荧光定量RT-PCR技术

real-time fluorescence quantitative RT-PCR detecting interfering effect of siRNA in HBV replication;

实时荧光定量RT-PCR技术检测siRNA对HBV复制的干扰效果

参考词条

复合实时荧光定量RT-PCR 实时荧光定量逆转录聚合酶链反应（RT-PCR）实时荧光定量PCR 实时荧光定量实时荧光定量-PCR 实时荧光定量RcR 实时荧光定量MSP 实时荧光定量 PCR 实时荧光定量（RQ-PCR）实时荧光定量分析 LightCycler实时荧光定量PCR Tagman实时荧光定量PCR 实时荧光定量PT-PCR 逆转录实时荧光定量PCR 组合注入交叉激发

补充资料：高压荧光灯用荧光粉

分子式：
CAS号：

性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO₄:Eu，Y(V，P)O₄:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO₄白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引 TaqMan实时荧光定量RT-PCR

1) real-time fluorescent quantitative RT-PCR 实时荧光定量 RT-PCR 2) real-time fluorescent quantitative RT-PCR 实时荧光定量RT-PCR 1. Expressions of bone metastasis genes in nasopharyngeal carcinoma by real-time fluorescent quantitative RT-PCR; 实时荧光定量RT-PCR检测鼻咽癌骨转移相关基因表达的研究 2. Establishment of a real-time fluorescent quantitative RT-PCR assay for the detection of chicken IFN-γ gene; 鸡γ-干扰素实时荧光定量RT-PCR检测方法的建立 3. Methods Using real-time fluorescent quantitative RT-PCR to analyze the expressions of DNMT1,DNMT3A and DNMT3B in normal liver cell line,pericacinoma cell line and hepatocellular carcinoma cell line respectively. 方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。 3) Real-time fluorescence quantitative RT-PCR 实时荧光定量RT-PCR 1. Methods After treatment of cultured 16HBE cells with NiSO_4 or NiSO_4 adding FJD drug serum,the variation of the free radicals and 8-oxo-dGTPase expression were detected by laser scanning confocal microscopy(LSCM) and real-time fluorescence quantitative RT-PCR,respectively. 方法体外培养的16HBE细胞分别经NiSO4暴露及NiSO4加扶正解毒汤含药血清共同处理后,采用激光扫描共聚焦显微镜(LSCM)及实时荧光定量RT-PCR检测自由基含量及8-oxo-dGTPase表达的变化。 2. Conclusion Real-time fluorescence quantitative RT-PCR used to detect the expression of . 目的建立实时荧光定量RT-PCR方法检测SurvivinmRNA的表达。 3. Based on the GenBank sequence data, one TaqMan-MGB probe and the corresponding primers were designed to construct a standard for real-time fluorescence quantitative RT-PCR to detect the expression of hsp70 in B. 为了探讨热激蛋白在B型烟粉虱Bemisiatabaci B-biotype抵抗高温中的作用,以含有B型烟粉虱热激蛋白基因hsp70片段的质粒为模板,用TaqMan-MGB探针和特异性引物构建了B型烟粉虱hsp70实时荧光定量RT-PCR检测体系,并检测了37℃~45℃高温胁迫及气温变化时B型烟粉虱成虫体内hsp70表达的情况。 4) real time RT-PCR 实时荧光定量RT-PCR 1. One step RT-PCR technique and Real time RT-PCR technique are applied in evaluating the correlation between rate of mRNA degradation in SD rats and postmortem intervals, especially in advanced stage PMI. 目的：本研究从基因库中筛选出两种稳定表达于不同个体与组织细胞中的看家基因GAPDH和β-actin，采用实时荧光定量RT-PCR技术研究大鼠死后不同时间和不同脏器中两种看家基因的mRNA降解规律，建立mRNA降解的程度与死亡时间关系的回归方程，探索用于晚期死亡时间推断较为理想的组织，并试图建立标准化的RNA提取与定量检测方法，希望能最终应用于法医学实践中。 5) Real-time RT-PCR 实时荧光定量RT-PCR 1. Establishment of Real-time RT-PCR for IFN-γ mRNA in Duck; 鸭IFN-γ mRNA实时荧光定量RT-PCR方法的建立 2. The CAT1 mRNA abundance was determined by real-time RT-PCR using SYBR Green I RT-PCR mix Kit. 以18S基因为内标,用实时荧光定量RT-PCR法(SYBR GreenⅠ试剂盒)检测CAT1 mRNA在60日龄长白猪十二指肠、空肠、回肠和结肠表达的组织特异性,以及在长白和蓝塘猪不同日龄其十二指肠、空肠、回肠表达的发育性变化。 3. The detection limit of the real-time RT-PCR assays was 10 RNA copies per reaction mixture. 分别设计HCoV-NL63和HCoV-HKU1特异的引物与荧光标记探针,并合成含靶基因的模板RNA,建立常规RT-PCR方法与实时荧光定量RT-PCR方法,对其灵敏性、特异性和可重复性以及用于临床样本的适用性等进行平行比较评价。 6) real-time fluorescence quantitative RT-PCR 实时荧光定量RT-PCR技术 1. real-time fluorescence quantitative RT-PCR detecting interfering effect of siRNA in HBV replication; 实时荧光定量RT-PCR技术检测siRNA对HBV复制的干扰效果参考词条复合实时荧光定量RT-PCR 实时荧光定量逆转录聚合酶链反应（RT-PCR）实时荧光定量PCR 实时荧光定量实时荧光定量-PCR 实时荧光定量RcR 实时荧光定量MSP 实时荧光定量 PCR 实时荧光定量（RQ-PCR）实时荧光定量分析 LightCycler实时荧光定量PCR Tagman实时荧光定量PCR 实时荧光定量PT-PCR 逆转录实时荧光定量PCR 组合注入交叉激发补充资料：高压荧光灯用荧光粉分子式： CAS号：性质：高压荧光灯中涂上Eu3+激活的钒酸钇或钒磷酸钇(YVO₄:Eu，Y(V，P)O₄:Eu)，可提高光效，改善显色性，增加了灯中的红色比和显色指数。YVO₄白色立方晶体耐温度猝灭，发光效率高，传递效率大，高压荧光汞灯主要用于街道，厂房和场地照明。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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