Objective To explore the optimized reaction conditions of semiquantitative fluorescence-based real-time MSP to detect CDH1 aberrant methylation of the lung cancer tissues.

A novel duplex real-time PCR assay for quantification of plasma DNA;

Real-time PCR Used to Detect p53 Gene Damage in Workers Exposed to Arsenic;

The method of preparation for the standard plasmids of real-time PCR and its application in the experiments;

Detection of adenovirus aerosols by real-time fluorescence quantitative PCR and PK15 cell infection rates;

Detection and application of 16S rDNA of Escherichia spp.by real-time fluorescence quantitative PCR;

Construction of plasmid standard for detecting Lateolabrax japonicus PPARα gene by real-time fluorescence quantitative PCR

Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR;

Application of gene microarray and real-time quantitative PCR in detecting human papilloma virus;

For exploring the relationship between gene expression of alpaca TYR gene family and alpaca s coat color,the relative expression quantity of TYR,TRP1,TRP2 in alpaca of different colors were analyzed by using real-time quantitative PCR in this research.