1) indirect ELISA
间接 ELISA
1.
ARV purified by differential centrifugation was chosed as coaten atingen of indirect ELISA,and the reaction conditions of ELISA was optimized: the coaten antigen was 252μg/ml and confined by 1%BSA 1.
本试验以差速离心纯化的鸡关节炎病毒(ARV)作为包被抗原,建立了检测 ARV 抗体的间接 ELISA 方法,该方法的最佳工作条件为:最佳抗原包被浓度为 252μg/ml,1%BSA 封闭1。
2) indirect ELISA
间接ELISA
1.
Recombinant BP26 and OMP31 proteins of Brucella used as the diagnostic antigen in indirect ELISA test;
重组布氏杆菌BP26蛋白和OMP31蛋白作为间接ELISA诊断抗原的研究
2.
Indirect ELISA in detection of antibodies in colostrums of the cows immunized with Helicobacter pylori;
间接ELISA法对H pylori免疫牛初乳中抗体的检测
3.
Detection of serum IgG and IgM antibodies againstCryptosporidium by indirect ELISA;
间接ELISA法检测血清隐孢子虫抗体IgG及IgM的实验研究
3) Indirect ELISA
间接ELISA法
1.
Using recombined SSB antigen expressed in Pichia Pastoris to detect anti-SSB antibody by indirect ELISA
利用酵母重组SSB抗原建立间接ELISA法检测抗SSB抗体的初步研究
2.
Methods Indirect ELISA was used to detect the changes of specific IgM and IgG4 antibodies in sera with acute Schistosomiasis,chronic Schistosomiasis and chronic Schistosomiasis after treatment for 12 and 24 months.
方法采用间接ELISA法检测急性血吸虫病、慢性血吸虫病及慢性血吸虫病患者治疗12月、24月后特异性IgM和IgG4的抗体变化。
3.
This fusion protein was used as antigen in indirect ELISA assay for the differential diagnosis of bovine tuberculosis,and comparison was made in indirect ELISA assays using PPD,TB10.
4与MPT83混合蛋白为抗原,用间接ELISA法鉴别牛结核病。
4) indirect enzyme-linked immunosorbent assay
间接ELISA
1.
An indirect enzyme-linked immunosorbent assay(indirct ELISA) was set up by purified antigen as coated antigen , HRP labeled rabbit anti-dog IgG and OPD as substract to decided: the specific IgG in sera from dogs .
以提纯抗原作为包被抗原,以HRP标记的免疫兔血清IgG作为酶标二抗,以邻苯二胺(OPD)为底物,成功建立了犬传染性肝炎血清抗体的间接ELISA检测方法。
5) ELISA
[英][i'laizə] [美][ɪ'laɪzə]
间接ELISA
1.
Study on Culture and Purification of Mycoplasma Ovipneumonia and Detection Antibody by Indirect ELISA;
绵羊肺炎霉形体的培养纯化研究与间接ELISA检测抗体方法的初步建立
2.
Establishment of Indirect ELISA for Equine Rhinopneumonitis and Serological Investigation in Xinjiang;
马鼻肺炎间接ELISA诊断方法的建立及血清学调查
3.
[Methods]1 797 women of child-bearing age were tested for anti-TORCH antibodies with indirect ELISA in four regions of Shanxi Province.
[方法]采用间接ELISA方法对山西省4个县区(中阳县、榆次城区、长治县、阳泉郊区)1 797名育龄妇女进行TORCH感染的血清学检测。
6) Indirect-ELISA
间接ELISA
1.
Development of an indirect-ELISA method for detecting specific antibody of canine distemper virus;
间接ELISA检测抗犬瘟热病毒抗体方法的建立
2.
Development of an indirect-ELISA for detection antibodies against canine distemper virus using recombinant N protein as antigen;
犬瘟热重组N蛋白抗原间接ELISA方法的建立及应用
3.
Development of an indirect-ELISA assay based on NP protein of goose paramyxovirus and detection of antibody levels in immunized geese;
鹅副黏病毒NP蛋白间接ELISA检测方法的建立及免疫鹅抗体水平的检测
补充资料：ELISA
分子式：
CAS号：
性质：属免疫标记技术。把已知抗原（或抗体）吸附到固相载体表面（常用聚苯乙烯微孔板），然的加入待检抗体（或抗原），再加入酶标记的抗体（或抗原），用洗涤的方法使固相载体表面只留下抗原抗体-酶复合物，最后加入底物，底物被酶催化变成有色产物。根据颜色的深浅定性或定量分析。特异性强，敏感度高，操作方便，广泛用于抗原或抗体的检测。
CAS号：
性质：属免疫标记技术。把已知抗原（或抗体）吸附到固相载体表面（常用聚苯乙烯微孔板），然的加入待检抗体（或抗原），再加入酶标记的抗体（或抗原），用洗涤的方法使固相载体表面只留下抗原抗体-酶复合物，最后加入底物，底物被酶催化变成有色产物。根据颜色的深浅定性或定量分析。特异性强，敏感度高，操作方便，广泛用于抗原或抗体的检测。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条