1) Indirect-blot ELISA
间接斑点ELISA
2) dot-ELISA
斑点ELISA
1.
Detection of Vibrio parahaemolyticus by Dot-ELISA;
应用斑点ELISA技术检测副溶血弧菌
2.
Valence of antibody was detected by dot-ELISA.
方法以30个氨基酸组成的多肽为半抗原与钥孔血蓝蛋白(mcKLH)通过碳化二亚胺(EDC)法化学偶联成完全抗原(多肽-mcKLH)免疫家兔;制备兔抗Cap4443多克隆抗体;斑点ELISA法检测抗体效价,经辛酸-硫酸铵法初步纯化抗体后,十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)电泳考马斯亮蓝检测其纯化后的纯度。
3) Dot-ELISA detection
斑点-ELISA 检测
4) indirect ELISA
间接ELISA
1.
Recombinant BP26 and OMP31 proteins of Brucella used as the diagnostic antigen in indirect ELISA test;
重组布氏杆菌BP26蛋白和OMP31蛋白作为间接ELISA诊断抗原的研究
2.
Indirect ELISA in detection of antibodies in colostrums of the cows immunized with Helicobacter pylori;
间接ELISA法对H pylori免疫牛初乳中抗体的检测
3.
Detection of serum IgG and IgM antibodies againstCryptosporidium by indirect ELISA;
间接ELISA法检测血清隐孢子虫抗体IgG及IgM的实验研究
5) Indirect ELISA
间接ELISA法
1.
Using recombined SSB antigen expressed in Pichia Pastoris to detect anti-SSB antibody by indirect ELISA
利用酵母重组SSB抗原建立间接ELISA法检测抗SSB抗体的初步研究
2.
Methods Indirect ELISA was used to detect the changes of specific IgM and IgG4 antibodies in sera with acute Schistosomiasis,chronic Schistosomiasis and chronic Schistosomiasis after treatment for 12 and 24 months.
方法采用间接ELISA法检测急性血吸虫病、慢性血吸虫病及慢性血吸虫病患者治疗12月、24月后特异性IgM和IgG4的抗体变化。
3.
This fusion protein was used as antigen in indirect ELISA assay for the differential diagnosis of bovine tuberculosis,and comparison was made in indirect ELISA assays using PPD,TB10.
4与MPT83混合蛋白为抗原,用间接ELISA法鉴别牛结核病。
6) indirect enzyme-linked immunosorbent assay
间接ELISA
1.
An indirect enzyme-linked immunosorbent assay(indirct ELISA) was set up by purified antigen as coated antigen , HRP labeled rabbit anti-dog IgG and OPD as substract to decided: the specific IgG in sera from dogs .
以提纯抗原作为包被抗原,以HRP标记的免疫兔血清IgG作为酶标二抗,以邻苯二胺(OPD)为底物,成功建立了犬传染性肝炎血清抗体的间接ELISA检测方法。
补充资料:ELISA
分子式:
CAS号:
性质:属免疫标记技术。把已知抗原(或抗体)吸附到固相载体表面(常用聚苯乙烯微孔板),然的加入待检抗体(或抗原),再加入酶标记的抗体(或抗原),用洗涤的方法使固相载体表面只留下抗原抗体-酶复合物,最后加入底物,底物被酶催化变成有色产物。根据颜色的深浅定性或定量分析。特异性强,敏感度高,操作方便,广泛用于抗原或抗体的检测。
CAS号:
性质:属免疫标记技术。把已知抗原(或抗体)吸附到固相载体表面(常用聚苯乙烯微孔板),然的加入待检抗体(或抗原),再加入酶标记的抗体(或抗原),用洗涤的方法使固相载体表面只留下抗原抗体-酶复合物,最后加入底物,底物被酶催化变成有色产物。根据颜色的深浅定性或定量分析。特异性强,敏感度高,操作方便,广泛用于抗原或抗体的检测。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条