2) GAD67-GFP transgenic mice
GAD67-GFP转基因小鼠
3) GFP transgenic mice
GFP转基因小鼠
1.
Methods Bone marrow cells isolated from GFP transgenic mice are directly planted in culture flask and an exchange of the total volume medium is made at different time.
方法分离GFP转基因小鼠骨髓细胞直接种植在培养瓶中,分别于2h、4h、6h、8h、10h、12h、14h、16h、24h和48h进行首次全量换液,培养3d后按细胞类型对贴壁细胞分别计数,并用CD44、CD45、CD54进行免疫细胞化学染色。
4) BMSCs of GFP transgenic mouse
GFP转基因小鼠BMSCs
1.
Objective: To construct the eukaryotic expression vector of human nerve growth factor beta by applying β-NGF gene and to explore the new method of cultivating and purifying BMSCs of GFP transgenic mice for further observing the expression pcDNA_3-β-NGF in BMSCs of GFP transgenic mouse .
目的:构建人β-神经生长因子基因(β-NGF)的真核细胞表达载体pcDNA_3-β-NGF,探讨绿色荧光蛋白(Green flurescent protein,GFP)转基因小鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)体外培养和纯化方法,进一步观察人pcDNA_3-β-NGF重组载体在GFP转基因小鼠BMSCs中的表达。
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