抑制消减杂交,suppression subtractive hybridization,在线英语词典,英文翻译,专业英语

1) suppression subtractive hybridization

抑制消减杂交

2) suppression subtractive hybridization(SSH)

抑制消减杂交

Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);

抑制消减杂交技术在鲤鱼抗寒研究中的应用

Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).

利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。

The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.

用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。

3) Suppression subtractive hybridization

抑制消减杂交法

Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K.

方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。

4) suppression subtractive hybridization

抑制消减杂交

Suppression subtractive hybridization coupled with gene chip technologies and their application;

抑制消减杂交与基因芯片技术及其联合应用

Cloning of differentially expressed genes of eosinophils from asthmatic patients by suppression subtractive hybridization;

应用抑制消减杂交克隆支气管哮喘病人嗜酸细胞差异表达基因

Suppression subtractive hybridization in construction of radiation-induced EST library;

运用抑制消减杂交构建辐射诱导EST库

5) suppressive subtractive hybridization

抑制消减杂交

The suppressive subtractive cDNA library was set up using suppressive subtractive hybridization(SSH).

方法提取胰腺癌和癌旁正常胰腺组织中的RNA和mRNA,应用抑制消减杂交技术(SSH)构建消减cDNA文库。

6) Suppression subtracted hybridization

抑制消减杂交术

参考词条

抑制消减杂交技术（SSH）抑制消减抑制杂交 PCR选择性抑制消减杂交抑制消减杂交cDNA文库抑制消减杂交文库抑制消减抑制消减文库杂交杂交 杂交-杂交瘤杂交种（杂交组合）杂交育种结构性态上部框架梁

补充资料：抑制杂交翻译

分子式：
CAS号：

性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

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1) suppression subtractive hybridization 抑制消减杂交 2) suppression subtractive hybridization(SSH) 抑制消减杂交 1. Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio); 抑制消减杂交技术在鲤鱼抗寒研究中的应用 2. Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH). 利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。 3. The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit. 用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。 3) Suppression subtractive hybridization 抑制消减杂交法 1. Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K. 方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。 4) suppression subtractive hybridization 抑制消减杂交 1. Suppression subtractive hybridization coupled with gene chip technologies and their application; 抑制消减杂交与基因芯片技术及其联合应用 2. Cloning of differentially expressed genes of eosinophils from asthmatic patients by suppression subtractive hybridization; 应用抑制消减杂交克隆支气管哮喘病人嗜酸细胞差异表达基因 3. Suppression subtractive hybridization in construction of radiation-induced EST library; 运用抑制消减杂交构建辐射诱导EST库 5) suppressive subtractive hybridization 抑制消减杂交 1. The suppressive subtractive cDNA library was set up using suppressive subtractive hybridization(SSH). 方法提取胰腺癌和癌旁正常胰腺组织中的RNA和mRNA,应用抑制消减杂交技术(SSH)构建消减cDNA文库。 6) Suppression subtracted hybridization 抑制消减杂交术参考词条抑制消减杂交技术（SSH）抑制消减抑制杂交 PCR选择性抑制消减杂交抑制消减杂交cDNA文库抑制消减杂交文库抑制消减抑制消减文库杂交杂交 杂交-杂交瘤杂交种（杂交组合）杂交育种结构性态上部框架梁补充资料：抑制杂交翻译分子式： CAS号：性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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