1) Suppression subtractive hybridization
抑制消减杂交技术
1.
Suppression subtractive hybridization in the cloning of gene fragments in relation to lung cancer metastasis;
抑制消减杂交技术克隆肺癌转移调控基因
2.
Research on cloning gene associated with lung cancer metastasis using suppression subtractive hybridization;
抑制消减杂交技术在肿瘤转移调控研究中的应用
3.
Suppression subtractive hybridization(SSH) is a good and highly effective method for gene cloning,which posses some character,such as convenient operation,high specificity,low background,good replication etc,and it can separate lower specific fragment.
抑制消减杂交技术(suppressionsubtractivehybridization,SSH)是基因克隆的新方法,具有简便易行、特异性高、背景低、重复性好、能分离出丰度较低的特异性片段等特点。
2) suppression subtractive hybridization
抑制性消减杂交技术
1.
Identification of genes associated with human osteosarcoma metastasis suppression using suppression subtractive hybridization;
应用抑制性消减杂交技术鉴定肿瘤转移抑制相关基因
2.
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
3.
1(-) transfected cells as the control to perform suppression subtractive hybridization (SSH).
1(-)空载体分别转染肝母细胞瘤HepG2细胞系,用抑制性消减杂交技术筛选pcDNA3。
3) Suppression subtractive hybridization(SSH)
抑制消减杂交技术(SSH)
4) Suppression subtracted hybridization
抑制消减杂交术
5) suppression subtractive hybridization(SSH)
抑制消减杂交
1.
Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);
抑制消减杂交技术在鲤鱼抗寒研究中的应用
2.
Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).
利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。
3.
The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.
用抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。
6) suppression subtractive hybridization
抑制性消减杂交
1.
Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;
抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.
Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;
抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.
Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;
应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
补充资料:抑制杂交翻译
分子式:
CAS号:
性质:又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实:以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质,包括一个特定的杂交mRNA,就能与未发生同DNA杂交的系统所合成的蛋白质相比较,其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号:
性质:又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实:以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质,包括一个特定的杂交mRNA,就能与未发生同DNA杂交的系统所合成的蛋白质相比较,其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条