1) one-step opposite orientation PCR
一步反向PCR法
2) TP-PCR
PCR一步法
1.
Construction of a Fused fpg Gene by Using TP-PCR Method;
PCR一步法构建融合蛋白基因fpg
3) inverse PCR method
反向PCR法
4) One-step RT-PCR
一步法RT-PCR
1.
Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor;
运用一步法RT-PCR检测柑橘鳞皮病毒及其在橘橙不同部位中的全年分布
2.
Research and Application of One-step RT-PCR Kit in the Detection of Tobacco Ringspot virus;
一步法RT-PCR检测烟草环斑病毒试剂盒的研制与应用
3.
Development of a one-step RT-PCR assay for detection of A subtype avian influenza virus;
禽流感病毒一步法RT-PCR检测方法的建立
5) one step RT-PCR
一步法RT-PCR
1.
E2 gene was amplified by one step RT-PCR from total RNA,which had been extracted from tissues of pigs doubting infected by classical swine fever virus and PK-15 infected by CSFV F114.
自疑似猪瘟病料及接猪瘟F114毒的PK-15细胞中提取总RNA,经一步法RT-PCR扩增E2基因。
2.
For detection of BPMV in imported soybean,the specific primers and Taqman probe were designed based on the coding gene of BPMV coat protein and one step RT-PCR and one step real-time fluorescent RT-PCR methods were developed.
针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。
6) one step duplex RT-PCR
一步法复合RT-PCR
1.
Development of one step duplex RT-PCR technique for detection of H5 subtype avian influenza virus;
H5亚型禽流感病毒一步法复合RT-PCR检测方法的建立
补充资料：反向PCR
分子式：
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
CAS号：
性质：一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA，得到某一含有已知序列的片段，将其自身连接环化，使该已知DNA两端的DNA序列连在一起，应用与已知DNA序列两末端同源的寡核苷酸为引物，可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反，故名。该法已有人用于染色体步读法(chromosome walking)。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条